Oocyte cryopreservation has encompassed various technical difficulties and thus remained a challenge to many cryobiologists. The effect of three widely used cryoprotectants and two cryopreservation techniques on the post thaw recovery rate of morphologically normal sheep oocyte, their fertilizability and developmental competence were analyzed. Ethylene glycol, propylene glycol and Dimethyl Sulf...

Oocytes cryopreservation represents a very promising tool for medicine and preservation of animal genetic resources. Assessment of oocytes viability after cryopreservation is often based on structural and developmental criteria whereas metabolism of thawed oocytes is not frequently studied. The aim of this study was to evaluate the impact of slow freezing and vitrification methods on the ATP co...

We evaluated the influence of two cooling rates (from 25 to 5 degrees C) on post-thaw function of frozen sperm in ocelots (Leopardus pardalis; n=3 males) and tigrinas (Leopardus tigrinus; n=4 males). Seven normospermic (>70% normal sperm) electroejaculates from each species were diluted with a 4% glycerol freezing medium, divided into two aliquots, and assigned to one of two cooling rates: fast...

The possibility to cryopreserve oocytes to be used in IVF treatment later in life has not only enlarged the reproductive options of cancer patients who are faced with gonadotoxic treatments, but also holds the promise of enlarging the reproductive options of healthy women whose personal circumstances (most often the absence of a partner) do not allow them to reproduce in their most fertile year...

The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving cat oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis. Germinal vesicle stage oocytes isolated from cat ovaries were either vitrified (n = 72) using a vitrific...

BACKGROUND Various approaches have been utilized in attempting to cryopreserve oocytes, beginning with slow cooling and more recently the advent of technique of vitrification. Now it seems that oocyte cryopreservation is no longer an experimental technique and it is being increasingly utilized in clinics around the world. As successful outcome in oocyte cryopreservation can be assessed by survi...

The first methods for fertility preservation (FP) were sperm freezing followed many years later by ovarian tissue freezing and oocyte vitrification. FP before cancer treatment is necessary because gametes are often destroyed at the same time as the cancer therapy kills malignant cells. It has been regularly offered to male cancer patients in the form of sperm cryopreservation. In female patient...

This proceedings report presents the outcomes from an international workshop designed to establish consensus on: definitions for key performance indicators (KPIs) for oocyte and embryo cryopreservation, using either slow freezing or vitrification; minimum performance level values for each KPI, representing basic competency; and aspirational benchmark values for each KPI, representing best pract...

The cryopreservation of female gametes is still an open problem because of their structural sensitivity to the cooling-and-freezing process and to the exposure to cryoprotectants. The present work was aimed to study the effect of vitrification on immature bovine oocytes freed of cumulus cell investment before freezing. To verify the feasibility and efficiency of denuded oocyte (DO) cryopreserva...

Fertility preservation is an important but often neglected need of cancer patients. There are several options available but many are considered experimental or are unproven. Most require interventions that can postpone the initiation of cancer treatment. The options include surgical procedures to move the ovaries out of the path of a radiation field, prophylactic medical therapy with gonadotrop...