De ondergrond is letterlijk het fundament voor ons bestaan. bevat drinkwaterbronnen, geeft draagvlak aan gebouwen, levert ecosysteemdiensten. In toenemende mate wordt de diepte gezocht, bijvoorbeeld als alternatieve bron energie. Het gebruik blijft echter sporadisch, autonoom, niet geordend en vooral zonder besef van wat menselijke interventies mogelijke consequenties hebben. vraag of gebruikt ...

Background: The ongoing healthcare transforma on results in systems medicine and personalized care. This requires advanced interoperability scenarios. Objec ves: The paper aims at introducing a reference architecture model to enable the harmoniza on of exis ng and future specifi ca ons and standards. Methods: For enabling the harmoniza on of standards and specifi ca ons, they have to be correct...

Antisense oligonucleotides are powerful tools for the in vivo regulation of gene expression. We have characterized the intracellular distribution of fluorescently tagged phosphorothioate oligodeoxynucleotides (PS-ONs) at high resolution under conditions in which PS-ONs have the potential to display antisense activity. Under these conditions PS-ONs predominantly localized to the cell nucleus whe...

To understand the parameters required for designing potent and specific antisense C-5 propynyl-pyrimidine-2'-deoxyphosphorothioate-modified oligonucleotides (C-5 propyne ONs), we have utilized a HeLa line that stably expresses luciferase under tight control of a tetracycline-responsive promoter. Using this sensitive and regulatable cell-based system we have identified five distinct antisense ON...

RATIONALE, AIMS, AND OBJECTIVES Improving the nutritional status of hospitalized patients has been shown to reduce length of stay (LOS), hospital costs, readmission rates, complication rates, and mortality. Provision of nutrient-rich, liquid, oral nutrition supplements (ONS) is one approach to improve nutritional status. Little information is available on ONS use and LOS among heart failure pat...

Oligonucleotides (ONs) modified with a locked nucleic acid (LNA) are widely used in the fields of therapeutics, diagnosis, and nanotechnology. There have been significant efforts towards developing LNA analogues bearing modified bridges to improve their hybridization ability, nuclease resistance, and pharmacokinetic profiles. Moreover, nucleobase modifications of LNA are useful strategies for t...

OBJECTIVES Patients with occipital neuralgia are often refractory to or intolerant of standard pharmacological and interventional management strategies. Although occipital nerve stimulation (ONS) may provide a unique alternative for such cases, a steep technical learning curve still exists. Lead migration (LM) is among the most challenging issues facing implanters performing ONS implantation. W...