During chemical refining steps (neutralization, bleaching and deodorization) of crude cottonseed oil (CSO), minor and major components, namely, tocopherols, sterols and fatty acids were evaluated by HPLC and GLC. HPLC analysis of total tocopherols (T) revealed that loss in these components were 7.12, 12.28 and 18.47 % in neutralized, bleached and deodorized oil respectively. On the other side, ...