A novel method for the direct analysis of condensed tannin components in bark was developed on the basis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a simple solid sampling technique. The MALDI mass spectra obtained from the wood (bark) powder sample clearly showed a series of peaks corresponding to the sodium ion adducts of condensed tannin oligome...

Contents of total polyphenols, condensed tannins and proanthocyanidins, and their stability to various pH values and temperatures were studied in Mexican blueberry, cuautecomate fruit, garambullo fruit, aubergine, coffee pulp and residues of black grapes. Several aqueous extracts, obtained through a one-pass-extraction process, were analyzed using liquid chromatography in order to quantify the ...

The effect of dietary condensed tannins (proanthocyanidins) on rat fecal bacterial populations was ascertained in order to determine whether the proportion on tannin-resistant bacteria increased and if there was a change in the predominant bacterial populations. After 3 weeks of tannin diets the proportion of tannin-resistant bacteria increased significantly (P < 0.05) from 0.3% +/- 5.5% to 25....

Condensed tannins were quantified in the barks of selected tree species, and antibacterial activity these substances against clinical subclinical isolates bovine mastitis evaluated. Tree from Mimosa tenuiflora, caesalpiniifolia, Anacardium occidentale, Stryphnodendron adstringens used, as well commercial tannin Acacia mearnsii. The extracted using a mixture ethyl alcohol acetone. moisture conte...

Tannins in forages complex with protein and reduce the availability of nitrogen to ruminants. Ruminal bacteria that ferment protein or peptides in the presence of tannins may benefit digestion of these diets. Bacteria from the rumina of sheep and goats fed Calliandra calothyrsus (3.6% N and 6% condensed tannin) were isolated on proteinaceous agar medium overlaid with either condensed (calliandr...

Bloat can be a serious problem for ruminant livestock grazing pastures of winter wheat (Triticum aestivum L.) in the southern Great Plains. Tannins in forage can reduce the incidence and severity of bloat. We measured the content and variability of total phenolics and condensed tannins (CT) in forage extracts from wheat varieties and experimental lines (ExpLines) to assess the feasibility of de...

Our objective was to determine if condensed tannin extract from quebracho trees (Schinopsis quebracho-colorado; red quebracho) could be used to reduce enteric methane emissions from cattle. The experiment was designed as a repeated 3 x 3 Latin square (4 squares) with 3 treatments (0, 1, and 2% of dietary DM as quebracho tannin extract) and 3 28-d periods. Six spayed Angus heifers (238 +/- 13.3 ...

The process called biohydrogenation occurs mainly in ruminant animals and during it, unsaturated fatty acids, and particularly poly-unsaturated ones (linoleic and linolenic) coverts to a saturated form of stearic acid. For many years, the beneficial effects of biohydrogenation intermediate fatty acids like cis-9 trans-11 linoleic acid, the main natural isomer of conjugated linoleic acids (CLA),...

Tannins gel was investigated as an adsorbent for heavy metal ions, such as lead, cadmium, copper, and others. The gel used in the present work was made by Mitsubishi Nuclear Fuel Co. Ltd. (MNF). It was found that the gel showed high adsorption capacity of heavy metal ions and the adsorption isotherms were correlated well by the Langmuir equation. The maximum adsorption capacity was influenced b...