Pseudomonas putida LP, which grows on lipoate, NH4NO3 and mineral salts, converts most of the organic substrate to bisnor-lipoate (1,2-dithiolane-3-propanoic acid) and acetyl-CoA. D-, L-, or DL-lipoate serve equally well as carbon and sulphur sources. There was no growth on or bacterial oxidation of the chemically synthesized bisnor- or tetranor-(1,2-dithiolane-3-carboxylic acid) chain-shortene...

Ring Opening Polymerization In article 2200359, Sonja Herres-Pawlis, Andreas Jupke, and co-workers show that the transition of new non-toxic catalysts for lactide ring opening polymerization from lab scale to industry is a highly complex process. Hence, promising end up in valley death between these scales. Model-based analysis can bridge this gap, giving an in-depth understanding pave way subs...

In 1944, Weinhouse et al. (1) reported that the incubation of carboxyllabeled octanoate with rat liver slices yielded acetoacetate in which the carboxyl and carbonyl carbons were equally labeled. Random condensation of 2-carbon units resulting from ,8 oxidation was therefore offered by these workers as a possible mechanism for the formation of acetoacetic acid from octanoate. The conversion of ...

Lipoic acid (Fig. 1) is a cofactor that is critical to the catalytic function of several key enzymes in intermediary metabolism that are found in all domains of life. There are two pathways to lipoyl-modified proteins. The de novo pathway from octanoylacyl carrier protein (ACP) involves the consecutive action of LipB and LipA (Fig. 2A), and the LplA-dependent salvage pathway activates and trans...

Ghrelin is a 28 amino acid, appetite-stimulating peptide hormone secreted by the food-deprived stomach. Serine-3 of ghrelin is acylated with an eight-carbon fatty acid, octanoate, which is required for its endocrine actions. Here, we identify GOAT (Ghrelin O-Acyltransferase), a polytopic membrane-bound enzyme that attaches octanoate to serine-3 of ghrelin. Analysis of the mouse genome revealed ...

FadD catalyses the first step in E. coli beta-oxidation, the activation of free fatty acids into acyl-CoA thioesters. This activation makes fatty acids competent for catabolism and reduction into derivatives like alcohols and alkanes. Alcohols and alkanes derived from medium chain fatty acids (MCFAs, 6-12 carbons) are potential biofuels; however, FadD has low activity on MCFAs. Herein, we gener...

* corresponding author. e-mail: [email protected] Phenethyl octanoate (PEO) is a natural flavour compound found in rum, spirits and wine. Six commercial and non-commercial lipase preparations were screened for 2-phenethyl octanoate synthesis activity, the most active enzymes were Lipozyme® RM IM and Palatase® 20000 L (immobilized and soluble Rhizomucor miehei lipases). Three substrates a...

We have performed a series of 10 ns Molecular Dynamics simulations of the sodium octanoate micelle in aqueous solution in the constant NpT ensemble, at p = 1 bar and T = 300 K. Two molecular topologies were studied, one with all internal degrees of freedom and the other constraining bond stretching and angle bending degrees of freedom. Two Lennard-Jones parameters for sodium ions, namely the OP...