Sequence variations exist at defined positions within genomes and are responsible for individual phenotypic characteristics, including a person's propensity toward complex disorders such as heart disease and cancer. As tools for understanding human variation and molecular genetics, sequence variations can be used for gene mapping, definition of population structure, and performance of functiona...

A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the ...