Objective: To compare the nucleated red blood cell (NRBC) counts of healthy and asphyxiated newborns as a key clinical indicator severity birth asphyxia short-term outcome. Methodology: This case control study was conducted at department neonatology Recep Tayyip Erdogan Hospital Muzaffargarh from June 2020 to November 2020. Study A total 112 term newborns, including 56 cases (with asphyxia) con...

Purine nucleotides occur in blood but their precursors and turnover rates have not been investigated systematically. Because blood can be incubated ilz vitro for several hours without much change in the purine nucleotides present, we have been able to determine the rate of incorporation of various radioactive precursors into blood nucleotides. In addition to studying these reactions in whole hu...

Fetal nucleated red cells which pass into the maternal circulation during pregnancy are a potential cell source for non-invasive prenatal genetic diagnosis. To sort these rare cells with a high degree of specificity, we focussed our attention on the erythropoietin receptor, a strictly erythroid-specific antigen. We first labelled these receptors with biotin-(sialyl)-erythropoietin, then isolate...

Background: Early diagnosis of perinatal asphyxia is very essential since it is a major cause of neurologic morbidity and mortality. The present study was undertaken to investigate the association between nucleated red blood cells count (RBCs) per 100 white blood cells (WBCs) and perinatal asphyxia with respect to its severity and prognosis. Materials and Methods: A prospective case-control stu...

To examine the limitations of reverse transcription (RT)-PCR for the detection of circulating tumor cells in blood, we established a RT-PCR for carcinoembryonic antigen (CEA). Whole blood (10(7) nucleated cells) was mixed with cells from the colon cancer cell line LS174T (concentrations ranging from 0 to 10(6) cells). RT-PCR was performed to detect CEA mRNA in blood under various conditions. He...

Erythroid enucleation is the process by which the future red blood cell disposes of its nucleus prior to entering the blood stream. This key event during red blood cell development has been likened to an asymmetric cell division (ACD), by which the enucleating erythroblast divides into two very different daughter cells of alternate molecular composition, a nucleated cell that will be removed by...

Cholesterol granulomas are composed of a distinct type of granulation tissue that contains lipids and cholesterol crystals. They are not believed to be a pathological entity, but rather an inflammatory tissue reaction to the presence of trapped blood secondary to localized hemorrhage.1 Cholesterol granulomas can be identified histologically by the presence of multi-nucleated giant cells, hemosi...