The indiscriminate use of pesticides for the last 40 years has almost eliminated natural enemies from many crop eco-systems. This scenario has led many countries to consider the potential of biological control as a component of pest management. At present, the world market for microbial pesticides is in excess of US $ 125 million per annum which is still less than 1 percent of the total global ...

Abstract Background Biological pesticides, especially baculovirus, often lose their activity under the influence of external light, temperature, and other changes. This limited application them. The present study was aimed to prolong biological ensure efficacy a pesticide using microencapsulation technology. Results In this study, gelatin/carboxymethylcellulose (CMC)- Spodoptera litura nucleopo...

The gene coding for the envelope (E) glycoprotein of dengue-2 virus was cloned into baculovirus (Autographa californica nuclear polyhedrosis virus). The recombinant virus contained the entire E protein gene, preceded by 38 nucleotides from the end of the prematrix glycoprotein gene and followed by the first 83 nucleotides of nonstructural protein 1. When expressed in Spodoptera frugiperda (Sf9)...

Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for the nucleocapsid protein gene of Hantaan virus. Two different cDNA clones representing the small genome segment of Hantaan virus were inserted into the transfer vector pAcYM1, and recombinants were generated by replacement of a portion of the baculovirus polyhedrin gene with the foreign, Hantaan virus...

Cellular functions known to involve actin are thought to occur in the cytoplasm. Even though actin has frequently been found in the nucleus, systems well-suited for studying the function of such nuclear actin are rare. We observed filamentous (F) actin within nuclei of IPLB-Sf-21 cells infected with Autographa californica M nuclear polyhedrosis virus (AcMNPV) as detected by laser confocal micro...

Sequence analysis of the p10 genes of three Bombyx mori nuclear polyhedrosis virus (BmNPV) isolates collected in Taiwan (Ta) and Japan (T3 and D1) showed that all possessed a deletion of an adenine residue, 210 bp downstream from the first base of the initiation codon when compared to the p10 gene of Autographa californica (multinucleocapsid) NPV (AcMNPV). This deletion caused a downstream term...