Assembly of a eukaryotic nucleus involves three distinct events: membrane recruitment, fusion to form a double nuclear membrane, and nuclear pore complex (NPC) assembly. We report that importin beta negatively regulates two of these events, membrane fusion and NPC assembly. When excess importin beta is added to a full Xenopus nuclear reconstitution reaction, vesicles are recruited to chromatin ...

Nuclear envelope assembly was studied in vitro using extracts from Xenopus eggs. Nuclear-specific vesicles bound to demembranated sperm chromatin but did not fuse in the absence of cytosol. Addition of cytosol stimulated vesicle fusion, pore complex assembly, and eventual nuclear envelope growth. Vesicle binding and fusion were assayed by light and electron microscopy. Addition of ATP and GTP t...

In this note, we propose, apparently for the first time, a new type of controlled nuclear fusion called ”intermediate” because occurring at energies intermediate between those of the ”cold” and ”hot” fusions, and propose a specific industrial realization. For this purpose: 1) We show that known limitations of quantum mechanics, quantum chemistry and special relativity cause excessive departures...

Evidence supporting cold fusion (LENR) is summarized and requirements an explanation must take into account are justified. A plausible nuclear-active-environment is identified by ruling out various possibilities and by identifying an environment that is common to all methods used to produce LENR. When this environment is combined with a plausible mechanism, many testable predictions result. The...

  Objective: Congenital fusion of jaws is a rare disorder which is observed in infants and cab ne syndromic and non-syndromic. Limited mouth opening in patients can affect the child growth and cause difficulties in feeding, swallowing and breathing.   Case: A In this study, a female infant from Afghanistan, settled in Qom referred to AlZahra maternity with upper and lower fusion jaws is reporte...

Studies at the Pacific Northwest National Laboratory (PNNL) in Richland, Washington, are being conducted to evaluate nondestructive examinations (NDE) coupled with mechanical testing of butt fusion joints in high-density polyethylene (HDPE) pipe for assessing lack of fusion. The work provides information to the Nuclear Regulatory Commission (NRC) on the effectiveness of volumetric inspection te...

Nuclear fusion in newly formed Chlamydomonas reinhardtii zygotes can be inhibited by drugs that affect microtubule stability, which include colchicine, amiprophosmethyl, oryzalin, and taxol. This inhibition can be monitored genetically by the production of haploid meiotic products from conjugations between haploid and diploid parents. Such zygotes would normally produce aneuploid progeny. Inhib...

Three reasons are given to dispute the claims of numerous experimenters that higher-thanexpected heat and radiation are obtained from nuclear fusion of deuterium atoms at room temperature: 1) the inability of two low-energy protons or deuterons to penetrate the mutual Coulomb barrier; 2) the production of heat in excess of that possible for the measured particulate radiation, and 3) the high le...