The etiology of psychotic disorders is still unknown, but in a subgroup patients symptoms might be caused by an autoimmune reaction. In this study, we tested patterns reactivity against potentially novel hippocampal antigens. Serum cohort 621 individuals with and 257 controls were first for on neuropil rat brain sections. Brain reactive sera (67 diseased, 27 healthy) further antibody binding to...

We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens...

Pre- and postimmunization sera from six malignant melanoma and six ovarian carcinoma patients were used to investigate the humoral immune response to antigens expressed in extracts of allogeneic tumor cells and lysates of these same cells infected with virus. Nitrocellulose paper replicas of cell extracts, fractionated by polyacrylamide gel electrophoresis, were used as antigenic targets. Antib...

AIMS To detect cytoplasmic and nuclear antigens using flow cytometry in acute leukaemia and to use this technique for double marker combinations. METHODS Cytoplasmic staining was carried out in samples from 40 cases of acute leukaemia with monoclonal antibodies against the myeloid antigen CD13, the lymphoid antigens CD3, CD22, mu chain and the enzymes terminal deoxynucleotidyl transferase (Td...

Fifty patients whose sera contained a speckled antinuclear antibody (ANA) were interviewed and examined to determine if there was any relationship between their clinical manifestations and the presence of certain serological markers. The results suggest that speckled ANA is usually found in patients with definite connective tissue diseases, but a significant minority have incomplete or early st...

BACKGROUND Autoimmunity is considered an uncommon but under-recognised cause of cognitive decline. METHODS Serum samples from 3,253 randomly selected subjects enrolled in the Hunter Community Study, aged 55-85 years, were assayed for thyrotropin stimulatory hormone, anti-thyroid peroxidase antibodies (TPO-Ab), anti-nuclear antibodies (ANA) and extractable nuclear antigens (ENA). Cognitive fun...

Epstein-Barr (EB) virus-immortalized B lymphocytes coexpress the EB viral latent gene products (EB viral nuclear antigens 1 to 6, the latent membrane protein and the terminal protein gene products) and the cellular activation antigen CD23. Immortalized B cells can be separated from those which are infected but not immortalized on the basis of CD23 expression as early as 2 days after in vitro in...

The nuclear distribution of DNA polymerase alpha and PCNA/cyclin in embryonic nuclei has been investigated, in a cell-free extract of Xenopus eggs that recapitulates a basic cell-cycle in vitro, by indirect immunofluorescence microscopy. Both antigens co-distribute with the chromatin in S-phase nuclei; however, as DNA replication is completed and nuclei progress into a G2 state anti-PCNA fluore...

background: inflammatory bowel disease unclassified (ibdu) is considered to be an aberrant immune response with loss of tolerance to many antigens. objective: this paper tries to address whether there is any value to test for auto-antibodies in such patients. methods: 60 patients with inflammatory bowel disease unclassified participated in the study. auto-antibodies to nuclear antigen, intestin...