The Prp19-associated complex [NTC (nineteen complex)] plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Only one component of the NTC, Cwc2, is capable of binding RNA. In the present study we report the 1.9 Å (1 Å=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. The Cwc2 core domain contains two sub-domai...

In this paper we propose a procedure for the storage and retrieval of digital signals utilizing DNA. Digital signals are encoded in DNA sequences that satisfy among other constraints the Noise Tolerance Constraint (NTC) that we have previously introduced. NTC takes into account the presence of noise in digital signals by exploiting the annealing between non-perfect complementary sequences. We d...

We have implemented the so-called λ-Zeeman technique (LZT) to investigate individual hyperfine transitions between Zeeman sublevels of the Rb atoms in a strong external magnetic field B in the range of 2500 − 5000 G (recently it was established that LZT is very convenient for the range of 10 − 2500 G). Atoms are confined in a nanometric thin cell (NTC) with the thickness L = λ, where λ is the r...

In this study, commercial-grade NiCr (80 wt % Ni, 20 wt % Cr) and NiCrSi (55 wt % Ni, 40 wt % Cr, 5 wt % Si) were used as targets and the sputtering method was used to deposit NiCr and NiCrSi thin films on Al₂O₃ and Si substrates at room temperature under different deposition time. X-ray diffraction patterns showed that the NiCr and NiCrSi thin films were amorphous phase, and the field-effect s...

OBJECTIVES To explore the immediate impact of the 2001 National Tobacco Campaign (NTC) advertising on movement towards quitting in a broadly representative sample of smokers. METHOD Repeated measures design with surveys two weeks apart. 1000 current smokers aged 18-40 were interviewed. 250 telephone interviews were conducted in each of Sydney and Melbourne (both unexposed at initial survey) a...

Near-threshold computing (NTC) has been shown to achieve maximal energy gains while maintaining performance metrics more favorable than subthreshold operation of circuits. Sensor subsystems are perfect candidates for NTC since low energy is often a crucial sensor system design desideratum and speed is rarely required. However, process variation affects threshold voltages (Vth), thus forcing hig...

Prp19 is the founding member of the NineTeen Complex, or NTC, which is a spliceosomal subcomplex essential for spliceosome activation. To define Prp19 connectivity and dynamic protein interactions within the spliceosome, we systematically queried the Saccharomyces cerevisiae proteome for Prp19 WD40 domain interaction partners by two-hybrid analysis. We report that in addition to S. cerevisiae C...