Dynein-ATPase is the intracellular motor for sperm motility. In the present work we assayed the dynein-ATPase activity in an axoneme-containing fraction of human sperm, free of plasma membranes, in normozoospermic and asthenozoospermic donors. Axoneme-containing fractions were isolated from semen samples obtained from healthy donors with either normozoospermia or asthenozoospermia, as indicated...

background and purpose: an important factor involved in infertility is reactive oxygen species (ros). ros can damage sperm dna, and involve lipid per-oxidation. ros elevation is under the influence of leukocyte activation. the objective of this study was to evaluate the level of ros as well as leukocyte, in normozoospermic (no) and ologoasthenoteratozoospermic (oat) ejaculates. materials and me...

background: use of cellular phones emitting radiofrequency electromagnetic field (rf-emf) has been increased exponentially and become a part of everyday life. this study aimed to investigate the effects of in vitro rf-emf exposure emitted from cellular phones on sperm motility index, sperm dna fragmentation and seminal clusterin (clu) gene expression. materials and methods: in this prospective ...

Background Fertility impairment may occur in men diagnosed with inflammatory arthritis. High disease activity, sex hormones imbalance and some modifying drugs are important factors considered to be involved this phenomenon [1]. Sperm parameters assessment is of key importance clinical practice. Studies focusing on fertility patients spondyloarthritides (SpA) showed discordant results Indeed, lo...

background: embryo selection is a vital part of in vitro fertilization (ivf) programs, with morphology-based grading systems having been widely used for decades. time-lapse imaging combined with embryo morph kinetics may proffer a non-invasive means for improving embryo selection. we report the first ongoing and chemical pregnancies using time-lapse embryo scope to select best embryos for trans...

Background: Infertility is estimated to affect up to 15% of couples of reproductive age, and male factors contribute to 50% of these cases. Globozoospermia syndrome is a severe form of teratozoospermia, with an incidence of less than 0.1%. It is characterized by round sperm head. The most prominent feature of globozoospermia is the malformation of the acrosome, which is totally absent in the to...

background: the lipids of the spermatozoa membrane are important for the fluidity and flexibility of spermatozoa. however, spermatozoa’s lipids are the main substrates for peroxidation, which may provoke severe functional disorder of sperm. objective: the aim of this study was to investigate the fatty acids composition of spermatozoa in men with asthenozoospermia, asthenoteratozoospermia and ol...

The study aimed to evaluate reversal of short- and long-term vas occlusion with reversible inhibition of sperm under guidance (RISUG) using dimethyl sulfoxide (DMSO) and sodium bicarbonate (NaHCO3) in male rabbits (Oryctolagus cuniculus). Animals were divided into seven groups containing five animals each. Fortnightly, semen analysis revealed that sperm concentration and output steadily decline...