The effects of calcium ionophores X-537A, A23187, and PR-47 on the action potential of canine cardiac Purkinje fibers were studied using standard microelectrode techniques. The ionophores hyperpolarize the membrane potential, eliminate the notch on the action potential plateau, decrease action potential duration, decrease plateau amplitude and duration, decrease spontaneous diastolic depolariza...

We designed a single-chain variant of the Arc repressor homodimer in which the beta strands that contact operator DNA are connected by a hairpin turn and the alpha helices that form the tetrahelical scaffold of the dimer are attached by a short linker. The designed protein represents a noncyclic permutation of secondary structural elements in another single-chain Arc molecule (Arc-L1-Arc), in w...

A novel formulation for combined scheduling and control of multi-product, continuous chemical processes is introduced in which nonlinear model predictive control (NMPC) and noncyclic continuous-time scheduling are efficiently combined. A decomposition into nonlinear programming (NLP) dynamic optimization problems and mixed-integer linear programming (MILP) problems, without iterative alternatio...

Pyruvate,Pi dikinase (PPDK, EC 2.7.9.1) and NADP-malate dehydrogenase (MDH, EC 1.1.1.82) were activated in the light and inactivated following a dark treatment in mesophyll protoplasts of maize. DCMU (up to 33 micromolar), an inhibitor of noncyclic electron transport, inhibited activation of MDH much more strongly than it did PPDK. Antimycin A (6.6-33 micromolar), an inhibitor of cyclic photoph...

12-0-tetradecanoyl-phorbol-13-acetate (TPA) can significantly reduce the Ca-ionophore-induced rise in the intracellular calcium concentration (Cai) of T lymphocytes measured by quin2 or fura-2 fluorescence. This counteraction of TPA is maximal at a preincubation of 90 min at TPA concentrations higher than 20 nM. 45Ca uptake and efflux measurements directly indicate that TPA does not activate th...

Abstrad Indudion of gene expression in response to calcium ionophores or thapsigargin, which inhibits the calciumATPase responsible for sequestering intracellular calcium, has frequently been attributed to dired stimulatory events subsequent to the elevation of intracellular free calcium. VL3O is a murine gene that is transcriptionally induced in response to a large array of mitogenic and trans...