Intracellular distributions of the low molecular weight RNA species of HeLa cells were determined by a nonaqueous method of cell fractionation, in which lyophilized cells were homogenized and centrifuged in anhydrous glycerol. The nonaqueous method was used to avoid artifactual extraction of weakly bound nuclear RNA during cell fractionation. We found that the mature small RNA species K, A, C, ...

Ferroelectrics with perovskite structure (ABO3), for example, lead titanate (PbTiO3), are the most studied ferroelectric oxides because of their versatile properties for use in thin film capacitors, electronic transducers, actuators, high-k dielectrics, pyroelectric sensors, and nonlinear optics. The studies on nanostructured PbTiO3 are limited, mainly due to the lack of facile synthetic techni...

Biophysikalische Chemie I Flüssigkeiten I Hohe Drücke I Magnetische Kernresonanz The concentration dependence of the activation volume AV* and the free activation enthalpy A G * for the inversion of the dimethylaminogroup has been determined in a series of dimethylamides in aqueous solution by high pressure H R N M R (p m a x = 150 MPa) . The compounds studied are the N,N-dimethylamides of form...

Kinetic parameters of the ammonolysis reaction of dichloroethane in nonaqueous media are studied and the optimum reaction conditions are reported. A suitable reactor for running the reaction under high pressure in the range of 50 to 500 °C is designed. Kinetic behaviours of the system is studied and other reaction parameters such as rate constant, kinetic order, and activation energy are de...

To ensure the reliability of amino acid analyses, the National Metrology Institute of Japan of the National Institute of Advanced Industrial Science and Technology (NMIJ/AIST) has developed high-purity certified reference materials (CRMs) for 17 proteinogenic amino acids. These CRMs are intended for use as primary reference materials to enable the traceable quantification of amino acids. The pu...

The structure of an ornithine-containing lipid from Z’Gobacillus fhiooxidans has been elucidated. Methanolysis of the lipid released methyl cis-ll,lZ-methylene-Z-hydroxyoctadecanoate. Acid hydrolysis of the residue yielded ornithine and a mixture of fatty acids, the major components of which were 3-hydroxyhexadecanoic and 2-hexadecenoic acids. Identification of the 3-hydroxy fatty acid was base...