نتایج جستجو برای: node explant
تعداد نتایج: 173850 فیلتر نتایج به سال:
Revolution in plant biotechnology can be categorized into in vitro culture and genetic transformation. Plant regeneration was successfully achieved in 1950’s while production of transgenic plant was accomplished in 80’s. For production of transformants, in vitro culturing strategies are prerequisite. Soybean has been cultured through organogenesis and embryogenesis but still it is considered re...
Objective: To compare the characteristics of human gingival fibroblasts (hGFs) obtained by tissue-explant and enzyme-digestion methods. Methods: The hGFs were isolated using tissue explants and enzyme digestion methods and identified by immunocytochemical analysis. Cell-surface molecules were detected by flow cytometry analysis. The colony-forming activity was examined by CFU-F assay. Oil red O...
:فعالیت قلب وابسته به ایمپالس های الکتریکی است که توسط سیستم ریتمیسیته تخصص عمل یافته ی تحریکی وهدایتی آن تولید وهدایت می شود. این سیستم نسبت به آسیب ناشی از بیماری های قلبی وداروهاومواد خاص حساس است.نتیجه ی اختلال در کار این سیستم غالبا پیدایش یک ریتم غیر عادی در الکتروکاردیوگرام می باشد. مطالعات در جانوران پیشنهاد میکند که کافئین در دوزهای بالاسیستم هدایتی قلب را متاثر میکند و بسیاری از پزشکا...
BACKGROUND Insulin-like growth factor 1 (IGF-1) activates prosurvival pathways and improves postischemic cardiac function, but this key cytokine is not robustly expressed by cultured human cardiac stem cells. We explored the influence of an enhanced IGF-1 paracrine signature on explant-derived cardiac stem cell-mediated cardiac repair. METHODS AND RESULTS Receptor profiling demonstrated that ...
OBJECTIVE The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique. MATERIAL AND METHODS Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were di...
Objective The purpose of this study was to investigate the effects of estradiol on the expression of hypoxia-inducible factor (HIF)-1α and the differentiation of trophoblasts in human first trimester villous explant cultures. Methods Villous explant cultures were established from first trimester human placentas (6-8 weeks of gestation, n=3). Normal villous tissues were explanted on Matrigel a...
A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledon...
Cerastium candidissimum Correns is an endangered species. Nevertheless, its ornamental characteristics make it ideal candidate for commercial use as a new and landscape plant. As there are no references to the in vitro propagation of this species, present research aims comparatively investigate potential that may have three different populations plant, from Mounts Parnassos (PS), Parnitha (PR),...
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