The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal horticultural interests. objective was to identify the culture media that support best organogenesis callogenic expressions four types aerial explants; nodal internodal segments leaf portions, by varying mineral composition twenty add...

Mouse embryos homozygous for a null mutation in nodal arrest development at early gastrulation and contain little or no embryonic mesoderm. Here, two Xenopus nodal-related genes (Xnr-1 and Xnr-2) are identified and shown to be expressed transiently during embryogenesis, first within the vegetal region of late blastulae and later in the marginal zone during gastrulation, with enrichment in the d...

considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

Nodal is a member of the transforming growth factor-β superfamily that plays crucial roles during embryogenesis. Recently, we have reported that Nodal inhibits trophoblast cell proliferation, migration and invasion, but induces apoptosis in the human placenta. In this study, we examined the regulation of Nodal by microRNAs. In silico analysis of Nodal 3'UTR revealed a potential binding site for...

dendrobium chrysanthum (orchidaceae) is highly valued in the ornamental industry and has important medicinal properties used in the preparation of herbal medicines in different parts of the world. however, this orchid species is getting depleted from its natural habitats due to unsystematic collections. therefore, in the present study, an efficient mass propagation protocol through direct shoot...

Asparagus racemosus belongs to the Asparagaceae family and genus is an endangered plant species that have been listed in red data book. For conventional breeding of racemosus, its tubers are used whichpossess a wide range antioxidants, leading wastage highly oxidative tuber roots. Using in-vitro propagation method we can propagate using nodal explants successfully only contamination-free enviro...

High frequency somatic embryogenesis and plant regeneration was achieved on calli developed from internodal and flower buds of E. foetidum. The explants were cultured on Murashige and Skoog medium supplemented with different concentrations and combinations of BA and IAA. The nodal explants produced multiple adventitious shoots while the internodal and flower buds induced callus, from which mult...

Asparagus racemosus (Shatavari) is a plant with immense medicinal values. It is reported to be recalcitrant to tissue culture practices. Sterile cultures were obtained when the explants were treated with (0.5%) Bavestine, Indophyl and 8HQC for 7 min and HgCl2 for 5 min.The present study on micropropagation of Asparagus racemosus through nodal explants showed better result in BAP (2 mg/l) along ...

A micropropagation protocol based on axillary bud proliferation has been developed from mature Lagerstromia parviflora adult tree. Nodal segments cultured on woody plant medium supplemented with 5.0 microl. BAP and 0.25 microm IAA gave maximum (86.9%) morphogenetic response. Proliferated shoots (10.7 per explants) were elongated to 3.9 cm within 6 weeks. In vitro produced micro-shoots were subj...