cyp3a4 probably has the broadest catalytic activity of any cytochrome p450. it is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. firstly, a total of 300 bp core distal enhancer of cyp3a4 xrem region (-7972/-7673) were amplified from human genomic dna. the pcr product was then ligated into a human secretory alkaline phosphat...

Rhizobium meliloti Nod(-) mutant WL131, a derivative of wild-type strain 102F51, was complemented by a clone bank of wild-type R. meliloti 1021 DNA, and clone pRmJT5 was recovered. Transfer of pRmJT5 conferred alfalfa nodulation on other Rhizobium species, indicating a role in host range determination for pRmJT5. Mutagenesis of pRmJT5 revealed several segments in which transposon insertion caus...

Phenotypic resistance to chloramphenicol and ampicillin was induced in sensitive Escherichia coli K-12 strains during incubation with the following substances: acetate, acetylsalicylate (aspirin), benzoate, dimethyl sulfoxide, 1-methyl-2-pyrrolidinone, and salicylate. In addition, acetyl-salicylate and salicylate induced resistance to nalidixic acid and tetracycline. The induction of resistance...

We have established the DNA sequence and analyzed the transcription and translation products of a series of putative nodulation (nod) genes in Rhizobium meliloti strain 1021. Four loci have been designated nodF, nodE, nodG and nodH. The correlation of transposon insertion positions with phenotypes and open reading frames was confirmed by sequencing the insertion junctions of the transposons. Th...

Concentration and time course dependence of two structurally related plant flavonoids naringenin and quercetin on the nodABC gene transcriptional activity (as reporter gene nodC-lacZ activity) in Rh. leguminiosarum bv. vicae strain D923 and respective growth, nodulation and nitrogen fixing responses of plant host (garden pea) to inoculation with pre-induced strains were studied. Naringenin was ...

We discuss in this paper the strong convergence for weighted sums of negatively orthant dependent (NOD) random variables by generalized Gaussian techniques. As a corollary, a Cesaro law of large numbers of i.i.d. random variables is extended in NOD setting by generalized Gaussian techniques.

With the goal of identifying changes in gene expression in CD4(+) T cells during the development of diabetes in the nonobese diabetic (NOD) mouse, we used DNA microarrays to analyze gene expression in CD4(+) T cells from the pancreatic draining lymph nodes of NOD/BDC 2.5 T cell receptor transgenic and WT NOD mice at different ages. At 4 and 6 weeks of age, we found up-regulation of a number of ...

We have produced a T-cell receptor (TCR) transgenic NOD mouse, 6.9TCR/NOD, in which the expression of both diabetogenic T-cells and naturally occurring autoantigen were simultaneously controlled. The parent T-cell clone, BDC-6.9, and T-cells from 6.9TCR/NOD mice recognize a currently unidentified antigen present in NOD but not in BALB/c islet cells. A gene that codes for the antigen, or a prote...