In previous investigations it was shown that in folic acid deficiency there is a large increase in the urinary excretion of formimino-n-glutamic acid (l-6). This increase has been explained by recent enzymatic studies demonstrating that the first step in the metabolism of formimino+glutamic acid is the transfer of the formimino group to tetrahydrofolic acid (7-9). Formimino-n-glutamic acid is a...

This paper reports on the yield and nutritional trends over 61 years as affected by various treatments (trashed, burnt with tops either left or removed and all plots either fertilised or not fertilised) of the burning and trashing trial (BT1) at Mount Edgecombe. The overall mean residual yield benefit due to trashing was 9.3 tc/ha/an after 39 crops. The capacity of the Arcadia soil form (Vertis...

We reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs) PfMSX binded to MSX consensus bind...

Since the first successful aggregation of mouse embryos was reported by TARKowsKi,ii) many studies on chimera production have been carried out.S'6'7) Chimeras produced by the aggregation of early embryos have been widely used in experimental investigations of mammalian embryolegy and developmental genetics.4'9) Chimeric experimental animals have also been used to study some problems of human ge...

A rapid screening (hydroponic) and field testing programme There is increasing interest in improving the nitrogen (N) use efficiency of cultivated crops, due to the high cost of synthetic N fertiliser. Since existing differences in N use efficiency between sugarcane varieties could be exploited to improve N fertilisation, a hydroponic pot experiment was conducted to test the performance of seve...

The modification-dependent restriction endonuclease AspBHI recognizes 5-methylcytosine (5mC) in the double-strand DNA sequence context of (C/T)(C/G)(5mC)N(C/G) (N = any nucleotide) and cleaves the two strands a fixed distance (N12/N16) 3' to the modified cytosine. We determined the crystal structure of the homo-tetrameric AspBHI. Each subunit of the protein comprises two domains: an N-terminal ...

The initial growth of the nacreous layer is crucial for comprehending the formation of nacreous aragonite. A flat pearl method in the presence of the inner-shell film was conducted to evaluate the role of matrix proteins in the initial stages of nacre biomineralization in vivo. We examined the crystals deposited on a substrate and the expression patterns of the matrix proteins in the mantle fac...

It has been clearly established (1, 2), through the use of N16-labeled material, that the utilization of free thymine for the synthesis of deoxyribonucleic acid (DNA) by the rat is extremely limited. However, these studies afforded but little information concerning the rate and extent of the metabolism of thymine. In the experiments reported here, thymine-204 has been found to be more rapidly a...