Mycoplasmas were searched in the ear canal flushing of 60 bovine in Brazil. The prevalence obtained was 80%. The percentages of typified species were 12.5%, for M. alkalenses; 2.1%, M. arginini; 8.35%, M. bovirhinis; 2.1%, M. bovis; 25.0%, M. conjunctivae; 14.6%, M. mycoides subsp. mycoides LC and 10.4% M. capricolum.

We describe the development and analytical validation of a 7-plex polymerase chain reaction assay coupled to a bead-based liquid suspension array for detection of multiple ruminant Mycoplasma spp. The assay employs a combination of newly designed and previously validated primer-probe sets that target genetic loci specific for Mycoplasma bovis, Mycoplasma mycoides cluster, Mycoplasma mycoides su...

BACKGROUND The three-dimensional structures of histidine-containing phosphocarrier protein (HPr), a member of the phosphoenolpyruvate:sugar phosphotransferase system (PTS), have been determined from Gram-negative and Gram-positive bacteria. The structure of HPr reported here for Mycoplasma capricolum is the first protein structure to be determined for this class of organism. Comparative structu...

i n a survey of pneumonia due to mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. pcr studies were confirmed the genus of mycoplasma although attempting for identification of strains m. mycoides, m. capricolum/caprine pleuropneumonia and m. arginin were in failure. the lesions i...

The energy requirements for fatty acid uptake by Mycoplasma capricolum were studied. Fatty acid transport and esterification to phospholipid appeared to be tightly coupled, since there was little intracellular accumulation of free fatty acid. Uptake was blocked by iodoacetate, n-ethylmaleimide, and p-chloromercuribenzoate. Glucose, glycerol, and potassium ions were necessary for fatty acid upta...