Mycobacterium sp. strain PYR-1 degrades high-molecular-weight polycyclic hydrocarbons (PAHs) primarily through the introduction of both atoms of molecular oxygen by a dioxygenase. To clone the dioxygenase genes involved in PAH degradation, two-dimensional (2D) gel electrophoresis of PAH-induced proteins from cultures of Mycobacterium sp. strain PYR-1 was used to detect proteins that increased a...

The in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. Gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin MIC90s, < or =1 mg/L, except 4 mg/L against methicillin-resistant Staphylococcus aureus and Enterococcus faecium). Gatifloxacin was two-...

atypical mycobacterium granulomatous skin infections are often accured by mycobacterium marinum, m. ulcerans, m. fortuitum, and m. avium colonies. skin infections probably originate from an environmental source such as contacting with aquatic animals, fish farming and swimming in the pools, and inoculate into skin through skin wounds, scratches, trauma, and surgery. the lesions appear as purple...

In fish spas, clients may submerge their hands, feet or whole body in basins with Garra rufa fish, for dead skin removal. Skin infections may result from using these spas, transmitted from fish to clients, through either fish or water, or from client to client. The microbiological water quality was determined in 24 fish spas in 16 companies in the Netherlands through analysis of a single water ...

Dr. Beatriz L. Gomez and colleagues described a new inhibition immunoassay for the diagnosis of histoplasmosis (1). My laboratory developed and extensively evaluated an antibody sandwich immunoassay for this purpose. It is important to contrast the two methods, as their performance characteristics differ. The characteristics of the assay developed in my laboratory are high sensitivity in dissem...

The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples coalho cheese sold Northeast region Brazil. Of analyzed samples, 35 were produced by artisanal process, using raw milk, 15 originated from industrialized establishments that pasteurize milk. Conventional real-time nested PCR for rv2807 gene M. tuberculosis complex performed directly samples. Samples grown si...