Two methods, based on analysis of the polymerase chain reaction-amplified 16S rRNA gene by restriction enzyme analysis (REA) or direct cycle sequencing, were developed for rapid identification of mycobacteria isolated from animals and were compared to traditional phenotypic typing. BACTEC 7H12 cultures of the specimens were examined for "cording," and specific polymerase chain reaction amplific...

OBJECTIVE Patients with cystic fibrosis are at great risk of infection by nontuberculous mycobacteria from the environment because of certain predisposing factors such as bronchiectasis, malnutrition, and diabetes. The aim of this study was to analyze the mycobacterial content of sputum smears and cultures from adult patients with cystic fibrosis attended at a specialized unit for adults from M...

Trypanosoma simiae, although similar to T. congolense in morphology and mode of development in the tsetse vector, is regarded as a separate species mainly because it is highly pathogenic to the domestic pig and fails to infect rodents. To establish whether the two species are distinct biochemically, we compared by isoenzyme electrophoresis 2 isolates of T. simiae with 7 stocks of T. congolense,...

OBJECTIVE To investigate for the presence of mycobacteria at water dams of Albaha, Saudi Arabia. METHODS An epidemiological study was conducted between 20 - 30 June 2013 in the Albaha region, Kingdom of Saudi Arabia. Five hundred and twenty samples (sand, wet stones, clay, and decayed vegetation) were collected from 13 dams in the region. These locations were: 3 dams from Alaqiq city (Thrad, ...