Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Nɛ-acetyl-lysine. Stable integration of transgenes encoding an engineered Nɛ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal...

As mice embryos develop, they undergo a stage of development called gastrulation [7]. The hallmark of vertebrate gastrulation [7] is the reorganization of the inner cell mass [8] (ICM) into the three germ layers [9]: ectoderm [10], mesoderm [11], and endoderm [12]. Mammalian embryogenesis [13] occurs within organisms; therefore, gastrulation [7] was originally described in species with easily o...

Open-field behaviour was recorded during 6-min forced exploration tests. In total, 483 tests were pedormed for following mice species: Apodemus agrarius, A.microps, A.sylvaticus, A.flavicollis, A.mystacinus, Mus musculus, M.domesticus and F1 hybrids of the last two species. Considerable variation among species was found for most behavioural patterns recorded. High level of number of squares cro...

Here we report a preliminary working scheme for the integrative application of transcriptomic, proteomic, and metallomic methodologies in environmental monitoring, by using as sentinel the wildlife species Mus spretus and as reference the gene/protein sequence databases from the key model species Mus musculus. We have demonstrated that the absolute transcript expression signatures quantified by...

BACKGROUND We have established a mouse model of spontaneous deafness by sib-inbreeding over 10 years. The mouse was designated as kuru(2) and has been previously reported in this Journal. MATERIALS AND METHODS In order to identify the genetic abnormality, the mouse was back-crossed to Mus musculus castaneus (CAST), and myosine 15 or myoXV on chromosome 11 was assumed to be the responsive gene...

The content of flavonoid compounds in betel leaf is used as a protection analgesic. purpose this study was to test the analgesic effects Banyuwangi green extract (Piper betle L.) on male white mice (Mus musculus). doses ethanol that were 4.696 mg, 9.392 mg and 18.784 positive control mefenamic acid. animals musculus) adapted for about 3 days experimental environmental conditions. Based results ...

Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studi...

The present paper deals with the piscicidal and muricidal (mice killing effect) activities of latex extract of Euphorbia royleana against freshwater snakehead fish, Channa punctatus and Swiss albino mice Mus musculus. Calculated values of lethal concentration (LC and LC ) showed that latex extract is toxic 50 90 against both the experimental animal. The toxicity of latex extract against freshwa...

INTRODUCTION Electrophoretic techniques by which mixtures of isoenzymes, and homologous proteins generally, may be resolved have become valuable tools in developmental biology. They make possible the study of development at the molecular level, in the sense that the differentiation of protein patterns underlies ontogenetic changes and tissue differences. The greater successes of this approach h...