Introduction: Amongst their distinct characteristics, human high-grade gliomas, particularly glioblastomas, exhibit not only rapid volumetric growth dynamics but also extensive local infiltration into the adjacent brain parenchyma. It is this characteristic local cell invasion that renders these tumors surgically non-curable and motivates ongoing research to better understand the crucial relati...

BACKGROUND The objective of this study was to evaluate the expression levels of glycolytic markers, especially hexokinase-2 (HK2), using a three-dimensional multicellular spheroid model of human ovarian adenocarcinoma (SKOV-3) cells and to develop an epidermal growth factor receptor-targeted liposomal formulation for improving inhibition of HK2 and the cytotoxicity of 3-bromopyruvate (3-BPA). ...

The blood brain barrier (BBB) has evolved unique characteristics such as dense coverage of the endothelial cells by pericytes and interactions with astrocytes through perivascular endfeet. We study BBB formation in a 3-dimensional multicellular spheroid system of human primary brain endothelial cells (hpBECs), primary pericytes (hpPs) and primary astrocytes (hpAs). We show for the first time th...

We have investigated the hypoxia Inducibility of vascular endothelial growth factor (VEGF) in multicellular tumor spheroids of HT29 cells using a monoclonal antibody to a fluorinated bioreductive drug, EFS [2-(2-nitro-lJI-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamidel, a chemical probe for hypoxia. We have shown that VEGF expression is predominantly localized in interior spheroid cell...

Multicellular spheroids are important tools for studying tissue and cancer physiology in 3D frequently used engineering as assembling units biofabrication. While the main power of spheroid model is mimicking physical-chemical gradients at microscale, real physiological environment (including dynamics metabolic activity, oxygenation, cell death, proliferation) inside generally ignored. At same t...

BACKGROUND Highly malignant gliomas are characterized by rapid growth, extensive local tissue infiltration and the resulting overall dismal clinical outcome. Gaining any additional insights into the complex interaction between this aggressive brain tumor and its microenvironment is therefore critical. Currently, the standard imaging modalities to investigate the crucial interface between tumor ...