background: absent in melanoma 2 (aim2) is an intracellular microbial dsdna sensor which plays an important role in production of proinflammatory cytokines through apoptosis associated speck-like protein containing a caspase activation and recruitment domain (asc) and caspase-1. micro-rnas (mirnas) play important roles in regulation of immune related genes. however, there is little information ...

In article number 2000307, Tim Blomeier and co-workers describe the Blue Light-Operated CRISPR/Cas13b-mediated mRNA Knockdown. “Lockdown” introduces optical control of RNA levels utilizing a blue light-dependent switch to induce expression CRISPR/Cas13b, which mediates sequence-specific knockdown exo- endogenous mRNA. Further, they combined tool with other optogenetic switches for efficient dow...

interleukin (il)-35 is a newly discovered suppressive cytokine and has been shown to alleviate  inflammatory  and  autoimmune  diseases.  the  purpose  of  this  study  was  to investigate immunomodulatory capacity of il-35 in patients with allergic asthma.il-35 mrna expression levels in peripheral blood mononuclear cells (pbmcs) were detected  by  quantitative  real-time  pcr  (qpcr).  the  fr...

In order to estimate responses of growth rate and growth hormone of Clarias gariepinus to stocking density, fish (initial weight 30.71 ± 0.89 g) were stocked in 120-L tanks at densities of 35, 65, 95, and 125 kg/m3 for 60 days. On day 30 and 60, the fish growth rates were measured, pituitary growth hormone (GH) mRNA levels and serum GH concentrations were examined by real-time qPCR and ELISA te...

hydrogen sulfide (h2s) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mrna expression of sodium bicarbonate cotransporter 1 (nbc1), trefoil factor1 (tff1) and trefoil factor2 (tff2) was not investigated. this study was aimed to evaluate the effect of h2s on mrna expression of nbc1, tff1 and tff2 in rat gastric mucosa in response to ...