Author contributions: D.U.L. and S.Y.K. performed cell-based assay experiments. D.U.L. and M.J.J. performed Ca measurement. J.Y.K. performed the statistical analysis. J.H.H. supervised and coordinated the study. J.H.H. and J.Y.K. wrote the manuscript. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted...

The present study investigated the effects of valerian methanolic extract and valerenic acid on expression LL-37 gene protein in A549 MRC5 line cells. After preparing Valerian seeds, sowing them March 2020, harvesting rhizome October was prepared from by maceration method. content determined using high performance liquid chromatography (HPLC). Two cell lines (A549 MRC-5) were used to extract, M...

An open reading frame with the characteristics of a glycoprotein-coding sequence was identified by nucleotide sequencing of human cytomegalovirus (HCMV) genomic DNA. The predicted amino acid sequence was homologous with glycoprotein H of herpes simplex virus type 1 and the homologous protein of Epstein-Barr virus (BXLF2 gene product) and varicella-zoster virus (gpIII). Recombinant vaccinia viru...

Abstract Telomere crisis contributes to cancer genome evolution, yet only a subset of cancers display breakage-fusion-bridge (BFB) cycles and chromothripsis, hallmarks experimental telomere identified in previous studies. We examine the spectrum structural variants (SVs) instigated by natural crisis. Eight spontaneous post-crisis clones did not show prominent patterns BFB or chromothripsis. The...

A bio-inspired multifunctionalized silk fibroin (BMS) was synthesized in order to mimic the interaction of nidogen with type IV collagen and laminin basement membranes. The designed BMS consists a motif ?-chain-derived, called IK peptide, covalently bound (SF) by using EDC/NHS coupling Cu-free click chemistry reaction, respectively. Silk chosen as main component because it is versatile biocompa...

TOPIC: Allergy and Airway TYPE: Original Investigations PURPOSE: Severe asthma is characterized by higher rates of airway hyperresponsiveness concomitantly increased eosinophilic neutrophilic systemic inflammation in which bradykinin plays a central role. Aerobic exercise (AE) the main component pulmonary rehabilitation inhibiting mild moderate asthmatics. In addition, AE mouse models allergic ...

Kratkosrochnaya ekspressiya genov yavlyaetsya odnim iz samyh shiroko ispol'zuemyh metodov v molekulyarnoj biologii, kak issledovatel'skih proektah, tak i dlya resheniya zadach biotekhnologicheskoj promyshlennosti. Nesmotrya na to chto sushchestvuyushchie kommercheskie transfekcionnye agenty pozvolyayut dobit'sya effektivnoj transfekcii vysokoj ekspressii celevyh kletkah, masshtabirovanie proizv...

Transient gene expression is one of the most common methods in molecular biology, equally relevant for basic research projects and biotechnological industries. Despite existence commercial transfection systems, which afford high efficiency levels reporter genes, expanding such systems to industrial scales often problematic due costs reagents. The well-described cationic calcium-phosphate are ac...

Pyrazinoic acid or pyrazine-2-carboxylic (PA), due to its nitrogenous heteroaromatic ring, can be explored as an anticancer agent. Here, a series of twenty novels PA derivatives have been synthesized and characterized using IR, NMR, mass spectrums. Their cytotoxic activity was evaluated against three different cancer cell lines, including lung (A549), breast (MCF-7), colon (HT-29). P16, the mos...