Abstract Background Influenza is a highly contagious respiratory virus with clinical impacts on patient morbidity, mortality and hospital bed management. The effect of rapid nucleic acid testing (RPCR) in comparison to standard multiplex PCR (MPCR) diagnosis treatment decisions unclear. This study aimed determine whether RPCR influenza MPCR was associated differences antibiotic antiviral (oselt...

Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vi...

We compare two fixed-prize mechanisms for funding public goods, an all-pay auction and a lottery, where public good provision can only occur if the participants’ contributions equal or exceed the fixed-prize value. We show that the provisional nature of the fixed-prize means that efficiency and endowment conditions must both be satisfied to assure positive public good provision. Our main findin...

OBJECTIVES The genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediat...

Multiplex PCR assays that can detect and identify three haemagglutinins and two neuraminidases of three main subtypes: H1N1, H1N2, and H3N2 of swine influenza virus (SIV), circulating in a pig population, were developed. Three oligonucleotide primer sets were evaluated based on the published sequences, with unique sizes characteristic for each subtype. The sequences of each primers were demonst...

We study two provisional fixed-prize mechanisms for funding public goods: an all-pay auction and a lottery. In our setting, the public good is provided only if the participants’ contributions are greater than the fixed-prize value; otherwise contributions are refunded. We prove that in this provisional fixed prize setting, lotteries can outperform all-pay auctions in terms of expected public go...

Aim: To determine the prevalence of Campylobacter jejuni and Campylobacter coli among broilers at the time of slaughter in and around Bareilly, India. Materials and Methods: A total of 100 chicken caecal samples were screened by conventional plating in modified charcoal cefoperazone deoxycholate agar with incubation at 42°C for 48 h under microaerophilic conditions. The characteristic colonies ...

Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and evaluated a one-step multiplex PCR (MPCR) for the straightforward differentia...

Bacteria is the main cause of genital disorder. Abortion, recurrent abortion and placenta retention are disorder accompanying with pregnancy failure. The current study aimed to find relationship between this Campylobacter infection species related For purpose, 50 aborted fetuses vaginal swabs were collected. Case history multiplex PCR applied. result showed that detected at a rate 26% in cases ...