objective(s) the main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. materials and methods metaphase spreads from 3 human-mouse cell lines (hf2x653, spmo-4 and f3b6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. metaphase chromosomes s...

the laboratory mouse is recognized as the pre-eminent model for genetic research. awareness of chromosomal patterns of experimental animals increases their value for a variety of different fields of study. we aimed to study mitotic chromosome preparations from nih, c57bl/6 and razi strains of mice, which are outbred, inbred and partially inbred laboratory mice respectively. bone marrow cells we...

objective: the lrr (leucine rich proteoglycans) is a molecular recognition motif found in proteins with some roles in cell adhesion, signal transduction, dna repair and rna processing. opticin is a member of this family. takanosu et al (2001) detected messenger rna expression of mouse opticin in the eye, heart, brain, testis, thyroid and epididymis by dot blot hybridization. in this study, exp...

introduction: the origin of neonatal oocyte development is unknown. however, estrogen plays an essential role during development of the female reproductive system. anastrozole is used as both ovulation stimulating and an anticancer drug. the aim of this study was to evaluate the impact of anastrozole on follicular development and differentiation in mice. materials and methods: in the present st...

background: tramadol narcotic drugs or epoxied artificial categories is weaker than morphine and stronger than ibuprofen and acetaminophen drug. the aim of this study was to investigate the effect of oral tramadol on some liver enzymes in the rat. materials and methods: in this study, 60 male wistar rats were randomized into three groups (one control group, and experimental groups of 1, and 2)....

the transcriptional factor sox2 regulates the expression of some of the developmental genes, which are essential for the maintenance of pluripotency of stem cells. sox2 also expresses in the female gamete during folliculogenesis, but its role remains ambiguous. the aim of this study was to investigate the expression of sox2 in the mice ovarian tissue during different stage of estrous cycle. adu...

respiratory virus infection is a major cause of asthma exacerbation. however, the underlying mechanisms of  this exacerbation  are unknown. therefore,  to  determine  the mechanisms, we examined the effect of influenza infection in a murine model of asthma.mice were divided into four groups: the phosphate-buffered saline (pbs), house dust mite (hdm), influenza, and hdm/influenza groups. the inf...

objective(s): in the current study, the effects of selected folk medicinal herbs were evaluated in d-galactose-induced aging in male mice.   materials and methods: male balb/c mice were randomly divided into 12 groups composing sham, control, and treated groups. aging was induced by administration of d-galactose (500 mg/kg/day for 6 weeks). a positive control group was assigned that received vi...

background: the basis of spermatogenesis is the spermatogonial stem cells (sscs). the concentration of sscs is very small. however, a system that supports the proliferation and maintenance of sscs in vitro could be used to preserve and expand sscs numbers as well as increase success in transplantation. it is a new avenue to restore spermatogenesis in azoospermia subjects. objective: proliferati...