The intrinsic growth, substrate uptake, and product formation biokinetic parameters were obtained for the anaerobic bacterium, Clostridium ljungdahlii, grown on synthesis gas in various pressurized batch bioreactors. A dual-substrate growth kinetic model using Luong for CO and Monod for H2 was used to describe the growth kinetics of the bacterium on these substrates. The maximum specific growth...

These experiments indicate that absorbance changes observed at the 425 nm isosbestic point of the Hb and HbCO following laser photolysis of HbCO provide a direct measure of the rates of quaternary conformational changes between rapidly reacting Hb (the immediate product of full photolysis) and slowly reacting normal deoxyhemoglobin. Hb, first observed by Gibson (Gibson, Q.H. (1959) Biochem. J. ...

The steady-state production of a product produced through the growth of microorganisms in a continuous flow bioreactor is presented. A generalised reactor model is used in which both the classic well-stirred bioreactor and the idealised membrane bioreactor are considered as special cases. The reaction is assumed to be governed by Monod growth kinetics subject to non-competitive product inhibiti...

In 1959, Jacques Monod wrote a manuscript entitled Cybernétique enzymatique [Enzymatic cybernetics]. Never published, this unpublished manuscript presents a synthesis of how Monod interpreted enzymatic adaptation just before the publication of the famous papers of the 1960s on the operon. In addition, Monod offers an example of a philosophy of biology immersed in scientific investigation. Monod...

The present study was aimed to evaluate the suitability of agro-wastes and crude vegetable oils for cost-effective production poly-?-hydroxybutyrate (PHB), growth kinetics PHB in Alcaligenes faecalis RZS4 Pseudomonas sp. RZS1 with these carbon substrates biodegradation accumulated by cultures. accumulates higher amounts corn (79.90% dry cell mass) rice straw (66.22% medium respectively. kinetic...

Push–pull tests are a popular technique to investigate various aquifer properties and microbial reaction kinetics in situ. Most previous studies have interpreted push–pull test data using approximate analytical solutions to estimate (generally first-order) reaction rate coefficients. Though useful, these analytical solutions may not be able to describe important complexities in rate data. This ...

We compared growth kinetics of Prorocentrum donghaiense cultures on different nitrogen (N) compounds including nitrate (NO3-), ammonium (NH4+), urea, glutamic acid (glu), dialanine (diala) and cyanate. P. donghaiense exhibited standard Monod-type growth kinetics over a range of N concentraions (0.5-500 μmol N L-1 for NO3- and NH4+, 0.5-50 μmol N L-1 for urea, 0.5-100 μmol N L-1 for glu and cyan...

As an introduction to my topic, let me quote from the 1970 bestseller, Chance and Necessity, by the late Jacques Monod. In this “essay on the natural philosophy of modern biology”, as the book is subtitled, the celebrated French biologist attempted, as many have done before and after him, to derive some sort of Weltanschauung from the science of his day. On the existence of intelligent life on ...

We have investigated the gating kinetics of calcium channels in the A7r5 cell line at the level of single channels and whole cell currents, in the absence and presence of dihydropyridine (DHP) calcium channel agonists. Although latencies to first opening and macroscopic currents are strongly voltage dependent, analysis of amplitude histograms indicates that the primary open-closed transition is...