there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

radiolabeled monoclonal antibodies, have created new innovations in diagnosis, research and therapy of disease in last 2 decades. one of the serious limitation of applications of radiolabeled antibodies in vivo is relatively low target to background activity. various strategies have been proposed to solve this problem including pre-targeting methods that was suggested in 1989. regarding importa...

hepatitis b virus (hbv) infection is the 10th leading cause of death worldwide. the most important diagnostic and screening marker for hbv infection is hepatitis b surface antigen (hbsag), and the most widely used hbsag screening test is enzyme-linked immunosorbent assay (elisa). in this study, an elisa assay has been developed for detection of hbsag using two novel monoclonal antibodies (mab) ...

background: light chain (lc) and heavy chain carboxyterminal subdomain (hcc) fragments are the most important parts of tetanus neurotoxin (tent) which play key roles in toxicity and binding of tent, respectively. in the present study, these two fragments were cloned and expressed in a prokaryotic system and their identity was confirmed using anti-tent specific polyclonal and monoclonal antibodi...

background: trastuzumab (herceptin) is a humanized monoclonal antibody (mab) which is used for specific treatment of metastatic breast cancer in patients with overexpression of her2/neu receptor. in this study, we have attempted to develop a biosimilar version of trastuzumab mab. methods: according to in silico studies, the heavy and light chains of trastuzumab mab were designed and constructed...

background: expression of epstein-barr virus latent member protein-1 (ebv lmp-1) and loss of p16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. thus, this study was conducted to examine the relationship between the loss of p16 and ebv lmp-1 expression in jordanian patients diagnosed with lymp...

alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

The presence of a low level of M protein in the peripheral blood <3gm/dl which is below the required cut off for the diagnosis of plasma cell myeloma is known as monoclonal gammopathy of undetermined significance (MGUS). Elevated levels of serum M protein and detection of M band on serum protein electrophoresis (SPE) can occur in chronic diseases not related to B cell disorders and not always d...

Background: Monoclonal antibodies have confirmed their merit as biotherapeutics across a wide spectrum of diseases, including cancer, heart disease, infection, and immune disorders. Materials and Methods: The dynamics of 89Zr-labelled monoclonal antibodies (MAb) after injection into the human body are modelled. This modified biokinetic model can be used for dose assessment not only for 89Zr-lab...