introduction: l. pneumophila is the most important cause of legionaires, disease, which is currently reported either as a nosocomial or community acquired infection. rapid and reliable diagnosis of legionella has hampered the epidemiological studies and disease control activities. in spite of good sensitivity and specificity, the culture method encountered serious shortcomings. polymerase chain...

objective: bacterial meningitis is a dangerous and sometimes fatal infection that affects the central nervous system. because some antibiotics can prevent some types of these bacteria and supress them from spreading and infecting, therefore it is important to know what type of virus or bacterium is causing meningitis. haemophilus influenzae and neisseria meningitides are the two main pathogens...

introduction: the main agents of sarcocystosis in cattle as an intermediate host include s. cruzi, s. hominis and s. hirsuta. a sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. case report: after collection of beef sample from yazd slaughterhouse, dna extraction was done with salting out method. the 18srrna gene as a specific target gene...

abrin known as ribosome inactivating protein’s (rip) it is a high cytotoxic plant protein. high lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. as a result, remaini...

equine piroplasmosis is a tickborne disease of equids with worldwide distribution, caused by theileria equi and babesia caballi. the aim of this study was molecular detection of t. equi and b. caballi in donkeys in northeastern iran and investigate the association between positivity of piroplasm infection and host-related factors. in the present study, blood samples were collected from 106 appa...

background and objectives: urinary tract infection (uti) is one of the most common infections and a major cause of patient morbidity world wide. the gold standard for detection of bacterial pathogens in uti is culture of urine specimens. in order to facilitate identification of bacterial pathogens in clinical urine specimens we designed a molecular method to detect bacterial pathogen in uti. ma...

background and objective : due to the evolution of multidrug-resistant strains, screening of natural resources, especially actinomycetes, for new therapeutic agents discovery has become the interests of researchers. in this study, molecular, chemical and biological screening of soil actinomycetes was carried out in order to search for peptide-producing actinomycetes. materials and methods : 60 ...

circulating tumor cells (ctcs) recognition and characterization in the peripheral blood of patients with breast cancer have proven practical and predictive value in different studies. however, the clinical significance of ctcs enumeration and molecular characterization in thepersonalization of breast cancer diagnosis and treatment remains under the debate. a literature search in pubmed, web of ...