Seven current methods of protein quantitation, Bradford (standard, micro, and 590/450 nm ratio), Lowry, bicinchoninic acid (BCA), UV spectrophotometry at 280 nm, and Quant-iT fluorescence-based determination, were compared with regard to their susceptibility to interferences due to the presence of suspended and not easily detectable clay particles. Bovine serum albumin (BSA) and Na-Wyoming mont...

Cultured skin fibroblasts were prepared from two unrelated adult patients with full expressions of Coffin-Lowry syndrome. Glycosaminoglycans (GAGs) were isolated either from the medium or from the cell layer of cultured skin fibroblasts. Two-dimensional electrophoresis of GAG preparations on cellulose acetate film revealed that hyaluronate was the major component both in the medium and in the c...

for comparison of the food value of four different epecies of aquatic weeds: potamogeton pectinatus , potamogeton lucens, potamogeton perfoliatus, najas minor and two species of terrestrial grass: medicago sativa and cynodon dactylon by grass carp (ctenopharyngodon ideda), was studied in the shahicl rejai fishfarm in sari (province of mazandaran). totally 6 concrete ponds with 30 cubic meter vo...

OBJECTIVES Exposure to latex allergens in latex gloves can cause occupational health problems in nurses, yet latex gloves are still widely used in Thai hospitals. Therefore, we conducted a study to determine the prevalence of latex sensitization in nurses and identify risk factors associated with sensitization. MATERIALS AND METHODS A questionnaire, providing information on personal character...

Some populations of Gundelia tournefortii were compared based on chemotaxonomical characteristics. In this study, some biochemical compounds such as total protein and storage proteins of shoot were studied. The populations were compared on the basis of biochemical characteristics using statistical methods. Different populations were collected from mountains of west Azarbaijan province. Total pr...

pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. this bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: a and b. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of p. aer...