A twin inhibition is observed for the esterification reaction between ethanol and isovaleric acid using immobilized lipase from Rhizomucor miehei in hexane and in mixed solvent system. The observed bi-substrate inhibition pattern follows a Ping-Pong Bi-Bi mechanism with dead-end inhibition of enzyme by both the substrates. An increase in Km value for alcohol in mixed solvent (0.645 M) than in h...

The reaction between lentil (Lens culinaris) seedling amine oxidase and its chromogenic substrate, p-dimethylaminomethylbenzylamine, has been studied by the stopped-flow technique. Upon being mixed with substrate in the absence of oxygen, the enzyme is bleached in a complex kinetic process. A yellow intermediate absorbing at 464 nm and the first product (aldehyde) are formed in subsequent steps...

In this paper, we study the equilibria of a physiological model describing the continuous culture in which two microbial populations compete for two substitutable resources. This work is an extension of the stability analysis of the phenomenological model of mixed microbial growth [M.M. Ballyk, G.S.K. Wolkowicz, Exploitative competition in the chemostat for two perfectly substitutable resources...

A simple method for the calculation of kinetic parameters (K(m), V(max)) under conditions of changing substrate concentrations is presented. An application of the method to detect shifts in groups involved in the utilization of a substrate in a mixed microbial culture is given.

Mixed disulphide formation in the presence of oxidized glutathione reversibly inactivates rabbit skeletal muscle aldolase. Inactivation is allosteric, preferentially modifying Cys-72 on the surface of the aldolase homotetramer distant from active-site locations and subunit interfaces. Ion-exchange chromatography fractionates partly inactivated aldolase into three distinct enzymic species: unmod...

In this study, aerobic digestion of tannery industry wastewater was carried out using mixed culture obtained from common effluent treatment plant treating tannery wastewater. The effect of pH, temperature, inoculum concentration, agitation speed and initial substrate concentration on the reduction of organic matters were found. The optimum conditions for COD reduction was found to be pH 7 (60%)...

−Biodegradation of methylene chloride by a mixed microbial culture, isolated from a common sewage treatment plant, was investigated in a batch system. Batch experiments were performed at room temperature (27 C) and pH value of 7. The methylene chloride concentration in growth media varied from 25 mgl to 250 mgl. A maximum observed degradation was 1 mglh at 100 mgl of methylene chloride. The cul...

Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

In this study, lead sulfide (PbS) films were grown on Fluorine-doped TinOxide (FTO) glass substrate by thermal evaporation in a horizontal furnace toinvestigate carrier gas effect on structural, morphological, elemental, optical, electricaland photovoltaic properties of PbS. X-ray diffraction (XRD) patterns confirmed theformation of cubic polycrystalline PbS particles fo...