In LPS-stimulated human neutrophils, engagement of the adenosine A2A receptor selectively prevented the expression and release of TNF-alpha, MIP-1alpha/CCL3, MIP-1beta/CCL4, MIP-2alpha/CXCL2, and MIP-3alpha/CCL20. In mice lacking the A2A receptor, granulocytes that migrated into the air pouch 4 h after LPS injection expressed higher mRNA levels of TNF-alpha, MIP-1alpha, and MIP-1beta than PMNs ...

Satiety cues a feeding animal to cease further ingestion of food, thus protecting it from excessive energy gain. Impaired control of satiety is often associated with feeding-related disorders such as obesity. In our recent study, we reported the identification of a neural pathway that expresses the myoinhibitory peptide (MIP), critical for satiety responses in Drosophila. Targeted silencing of ...

OBJECTIVE To characterize the envelope (Env) glycoprotein of HIV-1 in mother-infant pairs (MIP) that underwent near simultaneous or acute-phase seroconversion, we examined the Env sequence of the transmitted viruses and compare viral evolution within the pair. DESIGN Three MIP from a Zambian cohort that seroconverted at the same sampling time were identified and followed longitudinally. MET...

abstract background: molecular imprinting is a method for synthesizing polymers with structure-selective adsorption properties with applications such as, selectivity binding, drug delivery systems and anti-bodies. the present study aims at optimizing the preparation of molecularly imprinted polymer (mip) against l-phenylalanine, in order to increase phenylalanine-binding in enzymatic intestinal...

The fkpA gene of Salmonella typhimurium encodes a protein similar to the macrophage infectivity potentiator (Mip) proteins of Legionella pneumophila and Chlamydia trachomatis. Because Mip proteins enhance the ability of these intracellular pathogens to survive within macrophages and epithelial cells, we tested whether the product of the fkpA gene would have the same effect on the intracellular ...

We previously developed a mini-intronic plasmid (MIP) expression system in which the essential bacterial elements for plasmid replication and selection are placed within an engineered intron contained within a universal 5' UTR noncoding exon. Like minicircle DNA plasmids (devoid of bacterial backbone sequences), MIP plasmids overcome transcriptional silencing of the transgene. However, in addit...

Congenital cataract is both clinically diverse and genetically heterogeneous. To investigate the underlying genetic defect in three-generations of a Chinese family with autosomal dominant congenital cataracts, we recruited family members who underwent comprehensive ophthalmic examinations. A heterozygous missense mutation c.634G > C (p.G212R) substitution was identified in the MIP gene through ...

We report the complete genomic sequence of the murine haemopoietic stem cell inhibitor gene (SCI) (1). The SCI protein is identical to the previously identified macrophage inflammatory protein la (MlP-la) (1,2). Using a MIP-la cDNA clone (2), we isolated a single clone from a murine (DBA/2J) adult liver genomic library (Clonetech Labs., USA) which contains the complete SCI/MIP-la gene. The amin...

OBJECTIVE To investigate the correlation of macrophage inflammatory protein-1α (MIP-1α) gene single nucleotide polymorphisms (SNP) with the susceptibility to pigeon breeder's lung (PBL) in Chinese Uygur population. METHODS A total of 92 Uygur from Xinjiang, China were enrolled in the study. Among them, there were 32 patients with PBL, 30 negative controls with history of exposure to pigeons a...