Methylmalonyl-CoA epimerase (MCE) is an enzyme involved in the propionyl-CoA metabolism that is responsible for the degradation of branched amino acids and odd-chain fatty acids. This pathway typically functions in the reversible conversion of propionyl-CoA to succinyl-CoA. The Caenorhabditis elegans genome contains a single gene encoding MCE (mce-1) corresponding to a 15 kDa protein. This was ...

Alkaline protease from brinjal leaf (Solanum melongena) having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg. SDS-PAGE of the final preparation revealed a single protein band of approx 14 kDa. Purified enzyme was characterized and was successfully immobilized into the amorphous mesoporous silica (SBA-15) and crystalline mesoporous zeolite (Nano...

Fifty-two Holstein cows with different phenotypes of κ-casein (κ-CN) and β-lactoglobulin (β-LG) were selected to provide weekly milk samples for heating at 30, 70, 75 and 80°C for 2 min. Coagulating properties of heated milk samples measured as rennet clotting time, rate of curd firming and curd firmness at cutting were determined by a Formagraph. Milk samples were analysed for fat and casein. ...

Differences in clotting between human and cow's milk in the stomachs of infants are discussed . Gastric pH, after ingesting milk, of an infant up to 6 months of age stays at a pH range of 4-5, near the isoelec tric point of casein, and never reaches the value of 2, which is found in adults. Pepsin C (or gastricsin) can hydrolyze proteins at this pH range. Gastric emptying time is shorter with h...

Filamentous fungi possess unique features which make them attractive as hosts for the production of heterologous gene products. For example, certain fungal species are capable of secreting large quantities of protein in submerged culture. Selected strains of Aspergillus niger can produce greater than 20 g of glucoamylase I ~ I in industrial fermentations [ 11. Some filamentous fungi, including ...

One of the earliest biotechnological applications enzymes is cheese production. The high specific milk-clotting activity chymosin gives it an advantage over other proteases in industry. action based on hydrolysis bond between amino acid residues Phe105 and Met106 κ-casein molecule, which causes destabilisation casein micelles, coagulation milk its separation into a clot whey. A recombinant came...