Antonie J. W. G. Visser,*,† Petra A. W. van den Berg,† Nina V. Visser,† Arie van Hoek,† Harrold A. van den Burg,‡ Derek Parsonage,‡ and Al Claiborne‡ MicroSpectroscopy Centre, Department of Biomolecular Sciences, Wageningen Agricultural UniVersity, P.O. Box 8128, NL-6700 ET Wageningen, The Netherlands, and Department of Biochemistry, Wake Forest UniVersity Medical Center, Winston-Salem, North C...

Fluorescence-free microscopy: A new nonlinear optical microspectroscopy technique, femtosecond (fs) triple-resonance coherent anti-Stokes Raman scattering, in which the amplitude and phase of input fs laser pulses are optimally shaped to be in triple resonance with the molecular electronic and vibrational transitions, generates a coherent nonlinear signal beam at a new color with a highest poss...

We have performed FTIR transmission microspectroscopy on graphene oxide papers oriented with the nominal lattice planes parallel to the infrared optical axis. By polarising the IR light for samples of this geometry, spectral contributions of oriented oxide species are isolated from broad convoluted bands. Analysing the data alongside previous works, including experiments where samples were pert...

1 Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands 2 Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA 3 Laboratory of Biophysics, Wageningen University and Research, Wageningen, The Netherlands 4 Microspectroscopy Centre, Wageningen Un...

Protein secondary structures in human hair have been studied with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The CARS peak-shift mapping method has been developed and applied to hair samples with and without treatments by chemical reduction and mechanical extension. It clearly visualizes the treatment induced changes in protein secondary structures ...

Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major para...