The use of micropropagated trees has been widely extended, since micropropagation is a convenient technique of vegetative propagation that shows a number of interesting advantages, mainly for woody fruit trees. However, only few studies can be found in the literature on the field performance of micropropagated trees. In this work, the performance of different micropropagated fruit-tree rootstoc...

In vitro micropropagation of wild cherry is presently one of the main commercial ways to clonally propagate this species (Cornu and Boulay, 1986). In order to extend this technique to a large number of clones, it seems necessary to improve our knowledge of the behavior of the explants during the in vitro culture. Since plant growth regulators (PGR) play an important role in this technique (Marg...

Introduction: Squirting cucumber, Ecballium elaterium (L.) A. Rich. (Cucurbitaceae), is a wild medicinal plant found abundantly in the Mediterranean region. It has been utilized as a rootstock for many cucurbitaceous crops, mainly attributed to its resistance to abiotic as well as biotic stress (2). Important pharmacological uses (1, 9) are attributed to the bitter principles, cucurbitacins (5)...

Plants have been used for medicinal application ever since man has begun caring for his body and health. For centuries, the world has depended on the valuable properties of plant as a source of healing. Based on the considerable knowledge about these plant species from the ancient books, the modern systems of medicine have enabled these plants to find a place in the commercial MIcroProPAGAtIon ...

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm-3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm-3). Cultures were maintained in darkness. Soaking explants ...

A protocol for in vitro cloning of mature plants of Citrus limon using nodal shoot segments has been developed. Three to four shoots were induced per axillary meristems on MS medium amended with 9 μM BAP. Repeated transfer of the initial explants for up to six passages on MS medium [having 50% of the original concentrations of NH4NO3 and KNO3, and supplement of 250 mg L (NH4)2SO4] with 0.22 μM ...

aucuparia is an important slow growth forest tree with medicinal, industrial and ornamental uses which is applied for reforestation in high altitude of mountain lands. this species has been endangered at northern forests of iran; therefore micropropagation of adult trees by bud culture may help to reforestation. the best sterilizing treatment was the buds washing with hgcl2% 0.1 solution for 7 ...

strawberry is a valuable, nutritious, and economically important fruit all over the world including bangladesh. therefore, there is a demand to develop a suitable variety of strawberry. for this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto ms medium supplemented with different concentrations and combinations of 2,4-d, naa and ba for callus induction. the mo...

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3% (w/v) sucrose. The rate of multip...