background: potency test for control of rubella vaccine is a significant factor to qualify production line and vaccination pro­gram. for this reason, who recommends to use the microtitration method by both vaccine companies and control laborato­ries. then the study was done to improve this test. methods: three rubella virus samples, including an in-house standard, a lot of vaccine and an in- pr...

Cell adhesion assays are widely used to identify novel cellular ligands, novel cell surface receptors for these ligands and to elucidate the mechanisms responsible for the underlying cellular and molecular interactions. We report here the development of a novel centrifugal assay for fluorescence-based cell adhesion (CAFCA) that offers a number of advantages over the currently available assays. ...

Many biological assays have as their basis a link to the oxidative status of nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP). Many dehydrogenase enzymes use these coenzymes to transfer hydrogen groups between molecules. Because the reduced forms of these molecules differ from the oxidized forms in their ability to absorb light, it is possible to qua...

Antibiotic resistance has become a global health problem for humankind. Improper use of antibiotics resulted in the increasing evolved bacterial to them. There are different types mechanisms including efflux pumps. To overcome pump activity on drugs, combinatorial therapy existing antimicrobials with natural products is promising insight prevent multidrug resistance. In this study, inhibitory a...

A highly reproducible and sensitive real-time detection assay based on TaqMan technology was developed for the detection of hepatitis B virus (HBV) DNA and compared with two commercially available assays. The assay was validated with the Viral Quality Control panel, which also includes EUROHEP HBV DNA standards. This real-time PCR detection system had a dynamic range of 373 to 10(10) genome cop...

A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptinob mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptinob genotyping is necessary. To date, gel-based assays have been used for genotyping. Using the Invader Plus assay for single...

Many biological assays have as their basis a link to the oxidative status of nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP). Many dehydrogenase enzymes use these coenzymes to transfer hydrogen groups between molecules. Because the reduced forms of these molecules differ from the oxidized forms in their ability to absorb light, it is possible to qua...

Bead-based, multiplexing assays such as those provided by the Luminex xMAP technology, have proven to be highly useful for biomarker identification and quantification from cells, tissues and body fluids. Applications include biological research, drug discovery and clinical diagnostics. The original technology based on the use of polystyrene microspheres tends to have arduous, manual work flows ...

We describe here a field method we developed for colorimetry of erythrocytic acetylcholinesterase (EC 3.1.1.7) in capillary blood samples. Three stable, premixed assay reagents and de-ionized water (but no centrifuge or balance) are required. This method, adapted for a microplate format, is essentially that of Ellman et al. (Biochem Pharmacol 1961;7:88-95) as modified by George and Abernethy (C...