Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each...

We elucidate the detailed effects of gut microbial depletion on the bile acid sub-metabolome of multiple body compartments (liver, kidney, heart, and blood plasma) in rats. We use a targeted ultra-performance liquid chromatography with time of flight mass-spectrometry assay to characterize the differential primary and secondary bile acid profiles in each tissue and show a major increase in the ...

Almost immediately after a human being is born, so too is a new microbial ecosystem, one that resides in that person's gastrointestinal tract. Although it is a universal and integral part of human biology, the temporal progression of this process, the sources of the microbes that make up the ecosystem, how and why it varies from one infant to another, and how the composition of this ecosystem i...

Genome sequence data provide a framework for predicting potential microbial activities; however, the proteome content of the cell dictates its response to its environment. Microbiology is witnessing a major initiative to elucidate the nature of the proteome of large numbers of species. The tool driving the proteomic revolution is matrix-assisted laser desorption/ionization time-of-flight mass s...

Amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. However, no systematic study was performed about how the amphioxus defends against the microbial invasion. Here we reported a profile of gene transcription after Staphylococcus aureus (S.c) and Vibrio parahaemolyticus (V.p) challenged by suppression subtractive hybridization (SSH). When compared...

Radial oxygen loss (ROL) has been suggested to be a major process to protect plants exposed to the anaerobic by-products of soil anaerobiosis. The aim of the present study was to test the effects of root ROL from two submerged plants (Hydrilla verticillata and Vallisneria spiralis) on the rhizosphere oxygen profile and rhizosphere microarea. Phospholipid fatty acids (PLFAs) of sediment samples ...

Obesity is an increasing public health concern worldwide. According to the latest Organization for Economic Co-operation and Development (OECD) report (2014), the incidence of child obesity in Korea has exceeded the OECD average. To better understand and control this condition, the present study examined the composition of the gut microbial community in normal and obese adolescents. Fecal sampl...

PURPOSE To report the profile of microbial keratitis occurring after corneal collagen cross-linking (CXL) in keratoconus patients. METHODS A retrospective analysis of 2350 patients (1715 conventional CXL, 310 transepithelial CXL, and 325 accelerated CXL) over 7 years (from January 2007 to January 2014) of progressive keratoconus, who underwent CXL at a tertiary eye care centre, was performed....

a three-year field experiment was conducted under conditions of luvic chernozem soil. diversified nitrogen fertilization with 0, 60, 120, and 180 kg n ha-1, and microbial preparations improving soil properties were applied to potatoes, cv. ‘satina’, cultivation. n–no3 concentration in the soil profile (0–0.9 m) in autumn after potato harvesting fluctuated from 28 kg n ha-1 in the n0 treatment t...