Cell lines derived from type II lung cells were used to study interplays of substances affecting incorporation of labeled precursors [1(-14)C]palmitate and [methyl-3H]choline into phosphatidyl choline. Ethanol stimulated markedly biosynthesis of dipalmitoyl phosphatidyl choline in cloned rabbit lung cells; the stimulating action of ethanol was reduced very much by cortisol and less by ritodrine...

High glucose and fatty acid levels impair pancreatic beta cell function. We have recently shown that palmitate-induced loss of INS-1E insulinoma cells is related to increased reactive oxygen species (ROS) production as both toxic effects are prevented by palmitoleate. Here we show that palmitate-induced ROS are mostly mitochondrial: oxidation of MitoSOX, a mitochondria-targeted superoxide probe...

To test the hypothesis that regulation of palmitate metabolism, through carnitine palmitoyl transferase-1 (CPT-1) or through alterations of glycolysis, was involved in the pathway of palmitate-mediated cell death, cardiomyocytes were cultured from 7-day-old chick embryos. Palmitate-induced cell death, assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, was enhan...

Measurement of 13C-labeled fatty acid oxidation is hindered by the need for acetate correction, measurement of the rate of CO2 production in a controlled environment, and frequent collection of breath samples. The use of deuterium-labeled fatty acids may overcome these limitations. Herein, d31-palmitate was validated against [1-13C]palmitate during exercise. Thirteen subjects with body mass ind...

Objective: The aim of this study was to investigate the chemical compound antioxidant activity extract from andaliman fruit (Zanthoxylum acanthopodium DC.) and quality parameters total flavonoid determination highest activity.
 Methods: fruits were extracted using a solvent such as n-hexane, ethyl acetate 70% ethanol obtained extracts, follow 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. pe...

The synthesis of sugar fatty acid esters (SFAEs) from lignocellulosic biomass and oleic (C18:1) was catalyzed by immobilized-stabilized derivatives Candida antarctica lipase B in a methyl ethyl ketone medium. After steam-explosion pretreatment mixed hardwoods enzymatic hydrolysis at 15%wt solids, xylose glucose were purified/concentrated to mass ratio ~3 1. These sugars superior commercial as t...

The precise mechanism of uptake of long-chain fatty acids (FA) by cardiac myocytes is incompletely understood. We examined the involvement of sarcolemmal proteins in the initial uptake of FA by isolated rat cardiac myocytes, and the relation between initial uptake and metabolism. Cardiac myocytes were incubated in the presence of 90 microns [1-14C]palmitate complexed to 300 microns bovine serum...