Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble epoxide hydrolase (sEH). Herein, we demonstrate that ltx/iltx are toxic in a ...

Rat pancreatic juice cholesterol ester hydrolase (EC 3.1.1.13) has been purified 600-fold by sequential precipitation with 35 % acetone, diethylaminoethyl cellulose chromatography, and hydroxylapatite chromatography. The purified enzyme gives a single band on polyacrylamide disc electrophoresis and will not hydrolyze methyl butyrate, triolein, or secondary alcohol esters. Data are presented tha...

A significant portion of the beta-oxidized carbon skeleton of some polyunsaturated fatty acids can be recycled into de novo lipogenesis, i.e., cholesterol, saturates and monounsaturates. The recycling of carbon from linoleate was quantified in liver lipids of severely linoleate-deficient rats to determine whether it is more likely to be a function of redundancy or could be obligatory. After 13 ...