Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for K and A light immunoglobulin chains and CD3 antige...

In the recent paper on an improved technique for renal biopsies, Meadows and Schoemaker (1970) describe the use of a special wax to give fine sections at 1-2,.u. They illustrate their excellent results with Jones's silver technique. In this laboratory, however, difficulty was experienced with their use of ammoniacal ethanol and as we, in agreement with Eastham and Essex (1969), believe that the...

This report describes a case of granulomatous endometritis caused by coccidiosis in an immunologically uncompromised 63 year old patient. The glandular epithelium of the endometrium contained numerous intracytoplasmic cysts, corresponding to periodic acid Schiff positive and methenamine silver negative sporoblasts. The endometrial glands revealed reactive phenomena, such as eosinophilic and squ...

A 54-year-old man on systemic immunosuppressive therapy for 1 month after a liver transplant for hepatitis C virus presented with a 5-day history of a solitary plaque on the chest. Physical examination found an indurated, violaceous 53 3-cm tender plaque studded with pustules. Over the following days, the plaque became more purulent and developed a central erosion with necrosis (Fig 1). Punch b...

The histopathologic findings from a pustule specimen from the right arm showed a dense dermal neutrophilic infiltrate consistent with an abscess (Figure 2). Grocott methenamine silver (GMS) staining was performed and showed a few small budding yeast fragments (Figure 3). There was no overt evidence of herpes virus infection. Tissue culture yielded Scedosporium species. The histopathologic featu...

Aluminum chloride hexahydrate concentration-dependently inhibits perspiration and is highly effective in palmar and axillary hyperhidrosis. The main effect is in the sealing of the sweat glands, the precipitation of aluminum-mucopolysaccharide-complexes and the damage to the epithelial cells in the excretory ducts of the eccrine sweat glands result in sealing of these ducts. Other aluminum salt...

We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain ...