Yeast mRNA introns contain a conserved sequence, TACTAAC, required for splicing. We previously identified a putative splicing intermediate characterized by a stop to reverse transcriptase at the TACTAAC box of the wild-type rp51A (ribosomal protein 51A gene) intron. We now show that this stop is due to a branch and occurs at the identical nucleotide in the actin intron TACTAAC box. We show furt...

mRNA stability is modulated by elements in the mRNA transcript and their cognate RNA binding proteins. Poly(U) binding protein 1 (Pub1) is a cytoplasmic Saccharomyces cerevisiae mRNA binding protein that stabilizes transcripts containing AU-rich elements (AREs) or stabilizer elements (STEs). In a yeast two-hybrid screen, we identified nuclear poly(A) binding protein 2 (Nab2) as being a Pub1-int...

Circular RNAs (circRNAs) play key roles in several biological processes animals and their regulatory mechanism body color formation or pigmentation fish remains unclear. Here, circRNAs from black red individuals of Plectropomus leopardus were identified to clarify the competing endogenous RNA (ceRNA) network (circRNA-microRNA (miRNA)-messenger (mRNA)) formation. We detected a total 1,424 novel ...

As an approach to the study of yeast pre-mRNA splicing in vivo, we have examined properties of transcripts derived from a gal-UAS intron-containing fusion gene encoding RP51A and a series of its derivatives. RNA half-life measurements were carried out after transcription initiation was blocked by the addition of glucose. Pre-mRNA encoded by GalRP51A decayed with a half-life of approximately 6 m...

The present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats.Forty male rats were randomly divided into sham, control (I/R injury) and three crocin-pretreated groups. To induce I/R lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. Pretreated-rats rec...

The present study aimed to evaluate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats.Forty male rats were randomly divided into sham, control (I/R injury) and three crocin-pretreated groups. To induce I/R lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 3 h. Pretreated-rats rec...

Hypercholesterolemia is the primary risk factor for cardiovascular disease (CVD). Recent studies reported that stimulation of transintestinal cholesterol excretion (TICE), a nonbiliary excretion, can be strategy preventing CVD. Black elderberry (Sambucus nigra) has been to reduce CVD via its antioxidant, anti-inflammatory, and hypocholesterolemic effects. However, little known about role black ...

Pre-mRNA splicing is an essential step in gene expression that removes intron sequences efficiently and accurately to produce a mature mRNA for translation. It is the large and dynamic RNA-protein complex called the spliceosome that catalyzes intron removal. To carry out splicing the spliceosome not only needs to assemble correctly with the pre-mRNA but the spliceosome requires extensive remode...

STARMER, JOSHUA D. What can RNA hybrids tell us about translation?(Under the direction of Donald Bitzer, Mladen Vouk and Anne Stomp). Molecular biologists have been observing interactions between messenger RNA (mRNA) molecules and other non-coding RNA molecules for quite some time. Here I revisit some of the classical hybridizations between the 16S ribosomal RNA (rRNA) and mRNA during initiatio...

Transport of messenger RNA (mRNA) from the nucleus to the cytoplasm is an essential step of eukaryotic gene expression. In the cell nucleus, a precursor mRNA undergoes a series of processing steps, including capping at the 5' ends, splicing and cleavage/polyadenylation at the 3' ends. During this process, the mRNA associates with a wide variety of proteins, forming a messenger ribonucleoprotein...