research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

helicobacter pylori was the main cause of gastric cancer (gc) in all over the world that results in intestinal- and diffuse-type carcinoma. infection by this bacterium remains chronic for a long time. in vitro studies have highlighted the role of stem cells in gc. h. pylori infection results in tissue injury and the adult/tissue stem cells subsequently start to replace the dead cells. the genet...

background susceptibility-weighted imaging (swi) is extremely sensitive in the detection of superparamagnetic iron oxide (spio) nanoparticle-labeled cells. however, no study has compared molecular imaging for stem cell detection using swi and other mri pulse sequences. objectives this study aims to assess the sensitivity of swi in detecting spio nanoparticle-labeled, human bone marrow-derived m...

objective articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. on the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. little is known about the presence of mesenchymal stem cells in articular cartilage tissue. this subject is investigated in the present study. materials and methods articula...

background: mesenchymal stem cells (msc) are a very promising transplantable stem cell source for a variety of cell replacement therapies. as the main source of msc is bone marrow (bm), most of studies have been done on bm-derived msc (bm-msc). umbilical cord (uc)-derived msc (uc-msc) which are recently introduced, is one of the good alternative source for these cells. the objective of this stu...

this investigation aimed to examine the protective function of vitamin e on rat bone marrow mesenchymal stem cells (mscs) treated with cadmium chloride. rat bone marrow mscs were extracted using flashing-out and cultured in dmem containing 10% fbs and 100 u/ml pen/strep. at the end of the third passage, cells were divided into 4 groups including control, cadmium chloride, cadmium chloride + vit...

objective(s): hardystonite (ht) is zn-modified silicate bioceramics with promising results for bone tissue regeneration. however, ht possesses no obvious apatite formation. thus, in this study we incorporated sr and ti into ht to prepare sr-ti-hardystonite (sr-ti-ht) nanocomposite and evaluated its in vitro bioactivity with the purpose of developing a more bioactive bone substitute material. ma...

the importance of mesenchymal stem cells (mscs), as adult stem cells (ascs) able to divide into a variety of different cells is of utmost importance for stem cell researches. in this study, the ability of cartilage extract to induce differentiation of rat derived omentum tissue mscs (rot-mscs) into chondrocyte cells (ccs) was investigated. after isolation of rot-mscs, they were co-cultured with...

rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. the purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (mscs). five new zealand white male rabbits were used in the present study. a 2-mm hole was created in the a...