golestan province is one of the main tobacco growing regions in the country. tobacco streak virus (tsv) is a destructive pathogen on tobacco that has a wide host range and occurs worldwide. in order to serological detection of tsv at 13 region of golestan province, 500 infected samples belong to 8 families; chenopodiacae, amaranthaceae, fabaceae, solanaceae, malvaceae, poaceae, cucurbitaceae an...

An enzyme immunoassay (EIA) for anti-hepatitis B core (HBc) immunoglobulin G1 (IgG1) was compared with a commercial radioimmunoassay (RIA) for anti-HBc antibody (Corab: Abbott Laboratories, North Chicago, Ill.). In parallel tests of 445 consecutive samples, discrepant results were obtained with 2 samples, 1 of which was positive only by the RIA and the other of which was positive only by the EI...

Examples of both anti-M and anti-N have been reported in patients possessing these antigens; however, in only two instances has anti-M been reported as an autoantibody. This paper describes two additional patients with auto anti-M. In each case the anti-M was shown to be an autoantibody by absorption and elution studies. Optimum reactivity was obtained at 4 C. Column chromatogaphy and 2-mercapt...

conclusions due to the variable nature of antibiotic susceptibility patterns and etiological agents of septicemia, continual assessment of the most frequent pathogens associated with bloodstream infections and detection of their sensitivity patterns to locally available antibiotics seem to be reasonable measures. results from a total of 491 blood cultures, 74 (15.1%) samples were detected as po...

Using a direct agglutination test with 2-mercaptoethanol, 971 sera from farm animals and 71 sera from wild animals living in the Lublin province (Eastern Poland) were examined for IgG Toxoplasma gondii antibodies. Seropositive results were found in 27.5% of farm animals and in 19.7% of wild animals. Antibodies were more prevalent in cattle (53.7%), cats (50.9%), and dogs (42.2%) and less preval...

Serum samples from 697 Rocky Mountain bighorn sheep (Ovis canadensis) from North America were examined for antibodies to Toxoplasma gondii by the modified agglutination test incorporating mercaptoethanol and formalin-fixed tachyzoites. Antibodies to T. gondii were found in 25 of 697 (3.6%) sheep in titers of 1:25 (8 sheep), 1:50 (4 sheep), 1:100 (7 sheep), 1:200 (1 sheep), 1:400 (1 sheep), 1:80...

Antibodies to Toxoplasma gondii were determined in serum samples from 275 domestic cats from a mobile spay and neuter clinic from 8 counties in Ohio. The modified agglutination test incorporating whole formalinized tachyzoites and mercaptoethanol was used to determine antibodies. Antibodies to T. gondii were found in 133 (48%) out of 275 cats: in titers of 1:25 in 24, 1:50 in 37, and 1:500 or m...

In a previous paper, Tringali, Del Carpio, and Gismmanco (1966) reported that treatment of sera with 2-Mercaptoethanol (2-ME) caused a disappearance of non-syphilitic anticardiolipin reactivity, usually referred to as T'false positive" (EP) reactivity, as detected in a complement-fixation assay, However, similar treatment did not abolish the reactivity of syphilitic sera in the samie assay. Thi...