نتایج جستجو برای: mercaptoethanol

تعداد نتایج: 2124  

Journal: :Journal of bacteriology 1979
R E Hancock A M Carey

A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was determined by the solubiliza...

Journal: :The Journal of Biophysical and Biochemical Cytology 1960
Nancy L. R. Bucher Daniel Mazia

The earliest known event in the sequence leading to mitosis is the duplication of cell centers. The present investigation shows that the synthesis of DNA, although closely following it in time, is initiated entirely independently of this prior event. Fertilized eggs of the sea urchin, S. purpuratus, were exposed to beta-mercaptoethanol at intervals during development. This substance, when intro...

Journal: :Cancer research 1981
S Asano C Riglar

Colony-forming capacity of human melanoma cells was investigated in five established cell lines and isolated cells from seven metastatic or recurrent tumors. A single-cell suspension was added to 0.3% agar medium, and colonies were observed over 7 to 21 days. With the five cell lines, colony formation occurred in all cases and was usually accelerated by 2-mercaptoethanol, but there was wide var...

Journal: :The Journal of biological chemistry 1980
E K Barbehenn B T Kaufman

When chicken liver dihydrofolate reductase reacts with an excess of sodium tetrathionate, 1 mol of thiosulfate becomes covalently attached to the single sulfhydryl group of the enzyme. The identity of the thiosulfate was established by obtaining 1:l binding independently with both innerand outer-labeled [36S]tetrathionate. The thiosulfate form of the enzyme has 5to 10-fold more activity than n...

Journal: :The Journal of clinical investigation 1978
R B Counts S L Paskell S K Elgee

Human Factor VIII/von Willebrand factor, purified by calcium citrate-cellulose chromatography and 4% agarose gel filtration was subjected to sodium dodecyl sulfate gel electrophoresis on gels containing 2% acrylamide and 0.5% agarose. We find a series of multimers of which the apparent molecular weight of the higher members was congruent with5 million. The higher multimers were isolated by 2% a...

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