This report discusses two aspects of the development of a high-throughput separation technique aimed at separating cells based on phenotypic differences in membrane composition using dielectrophoresis (DEP): automated particle characterization and electrodeless DEP separations. First we discuss an automated technique to measure cellular dielectrophoretic mobility as a function of frequency. Sec...

The mitochondrial compartment of budding yeast (Saccharomyces cerevisiae) is a highly dynamic net-like structure of tubules that constantly undergo fusion and fission. The outer membrane protein Fis1p plays a crucial role in mitochondrial fission. Here we report on the temporal and spatial dynamics of this organelle in wild-type cells and in fis1Delta mutants. Mitochondria of fis1Delta mutants ...

Membrane-based separations are an attractive means to separate hydrogen from gas mixtures in order to use hydrogen in energy-related applications. Zeolite membranes are robust materials that are well suited to be used in harsh conditions, but they are not typically selective for hydrogen. Several studies have shown that highly selective separations of hydrogen are possible using zeolite membran...

Separating oil-water mixtures is critical in a variety of practical applications, including the treatment industrial wastewater, oil spill cleanups, as well purification petroleum products. Among various methodologies that have been utilized, membranes are most attractive technology for separating emulsions. In recent years, selective wettability attracted particular attention separations. The ...

The modern challenges for membrane separations in a wide range of processes require more sophisticated approaches for the detection and remediation of fouling, i.e., the association of solutes, particulate matter, and colloids on and/or within a membrane. Most commonly, fouling is assessed from inferred measurements of permeation rate and/or permeate quality. The use of acoustic techniques for ...

time-dependent schrödinger equation for a 1-d model of hydrogen molecular ion h2+ in intenselaser field linearly polarized along the molecular axis is solved. ionization rates are calculated for differentinitial states. the evolution of electronic wavefunction at fixed inter-nuclear separations are simulated andanalyzed. the results obtained for the ground state of this 1-d model of h2+ show ap...

Membrane gas separation at high temperatures and in the presence of aggressive chemicals, such as H2S, NH3, SOx, NOx hot water vapor, is attracting interest from both a fundamental industrial standpoint. To fully exploit benefits membrane technology, it imperative to develop materials that withstand thermally chemically harsh conditions, well understand principles underpinning transport species...

Since membrane separations, such as vapor permeation (VP) or pervaporation (PV), are not limited by vapor-liquid equilibrium, their coupling with distillation can exert a synergistic effect, overcome the thermodynamic limits of distillation, and improve energy efficiency, especially suitable for separation azeotropic mixtures that otherwise requires special processes. Therefore, there is an inc...