The growth response of Lactobacillus leichmannii to vitamin B12 has been studied extensively. Investigators have used semisynthetic media (Skeggs et al., 1950; Lees and Toothill, 1952) or basal media supplemented with tomato juice or liver extracts (Peeler et al., 1949; Schweigert et al., 1950). Recently Skeggs and her associates (1953) prepared a synthetic medium and studied the nutritional re...

Ten dental casting alloys were tested for alloy-element release into cell-culture medium, and this release was related to alloy composition, alloy microstructure, and alloy cytotoxicity (previously determined). Cell-culture medium was analyzed for alloy elements by flame atomic absorption. Concentrations of elements in the medium were normalized by dividing them by their atomic abundance in the...

In the present study, nine compounds (1-9) were isolated from Talaromyces wortmannii LGT-4 (anendophytic fungus from Tripterygium wilfordi) which was cultured in CYM Medium. Their structures were determined as 4-hydroxyphthalide (1), Fumitremorgin C (2), Ergosterol (3), 3-(2-hydroxypropyl)-8-h...

objective: n present study effect of various factors including sucrose, agar, peg, agno3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties dom siah and nemat. methods: 330 explant (mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction murashige and skoog (ms) medium (murashige and skoog 1962) s...

The effects of growth in media supplemented with lipid-depleted fetal calf serum (LDS-media) on morphology, saturation density, and lipid composition were studied in Balb/c3T3, SV3T3, and Concanavalin A selected SV3T3 revertant cells (SV3T3 Rev cells). Cells grown in media containing complete fetal calf serum (FCS-medium) or reconstituted FCS (RS-medium) were used as controls. Growth in LDS-med...

The composition of a culture medium is one of the most important parameters to be analyzed in biotechnological processes with industrial purposes, because around 30-40% of the production costs were estimated to be accounted for the cost of the growth medium [1]. Since medium optimization using a one-factor-at-a-time approach is time-consuming, expensive, and often leads to misinterpretation of ...

We report new high resolution measurements of the elemental and isotopic composition of galactic cosmic ray B, C, N, and 0 nuclei with ~ 30 to ~ 130 MeV nucleon-•. These observations place limits on the isotopic composition of the cosmic ray source and restrict possible models of cosmic ray origin and propagation. In particular, we find that N is significantly depleted in the cosmic ray source ...

In the present study, nine compounds (1-9) were isolated from Talaromyces wortmannii LGT-4 (anendophytic fungus from Tripterygium wilfordi) which was cultured in CYM Medium. Their structures were determined as 4-hydroxyphthalide (1), Fumitremorgin C (2), Ergosterol (3), 3-(2-hydroxypropyl)-8-h...