نتایج جستجو برای: meca

تعداد نتایج: 1758  

2014
Roxana SAHEBNASAGH Horieh SADERI Parviz OWLIA

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) is the main cause of hospital infection emerged over the last decades. Rapid detection of MRSA is important for patient care and proper usage of infection control. Detection of mecA genes (encoding resistance to methicillin and other similar antibiotics) and nuc genes (encoding staphylococcal thermostable nuclease) by PCR method is n...

Journal: :American journal of physiology. Lung cellular and molecular physiology 2002
Manako Taira Jun Tamaoki Kazuyuki Nishimura Junko Nakata Mitsuko Kondo Hisashi Takemura Atsushi Nagai

To examine the effect of adenosine A(3) receptor stimulation on airway mucociliary clearance, we measured transport of Evans blue dye in rabbit trachea in vivo and ciliary motility of epithelium by the photoelectric method in vitro. Mucociliary transport was enhanced dose dependently by the selective A(3) agonist N(6)-(3-iodobenzyl)-5'-N-methylcarbamoyladenosine (IB-MECA) and to a lesser extent...

2014
Min-Hee Kang Min-Joo Chae Jang-Won Yoon Seung-Gon Kim So-Young Lee Jong-Hyun Yoo Hee-Myung Park

The prevalence, virulence potential, and antibiotic resistance of ophthalmic Staphylococcus pseudintermedius (SP) isolated from dogs were examined. Sixty-seven Staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the Staphylococcus intermedius group (SIG) nuclease gene (SInuc), exfoliative toxin gene for SIG (siet), and antibiotic resistanc...

Journal: :Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia 2015
G Horna L Astocondor J Jacobs C García

BACKGROUND Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS Thre...

Journal: :Journal of clinical microbiology 1999
A van Griethuysen M Pouw N van Leeuwen M Heck P Willemse A Buiting J Kluytmans

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methi...

Journal: :Saudi medical journal 2009
Ihab Moussa Atef M Shibl

OBJECTIVE To examine the recovered strains phenotypically, by conventional methods and genotypically by polymerase chain reaction (PCR), for direct detection of Staphylococcus aureus (S. aureus) 16S ribosomal Ribonucleic Acid (rRNA) gene (which serves as an internal control) and mecA gene. Secondly, introduce multiplex PCR targeting at the same time S. aureus 16S rRNA, Panton-Valentine Leucocid...

Journal: :Enfermedades infecciosas y microbiologia clinica 2004
Maria João Soares Carlos Soares Ana Constança Mendes Maria Luís Guimarães José Manuel Cabeda José Manuel Amorim

We evaluated three rapid methods to detect methicillin-resistant Staphylococcus aureus (MRSA) and compared them with PCR amplification of mecA. A total of 103 S. aureus strains were studied by MRSA-Screen, BBL Crystal, Velogene Genomic and mecA PCR. All the methods detected the 61 MRSA strains having the mecA gene, showing 100% sensitivity and specificity. Despite the correlation between all th...

Journal: :Journal of clinical microbiology 2012
Senda Mezghani Maalej Faouzia Mahjoubi Rhimi Marguerite Fines Basma Mnif Roland Leclercq Adnene Hammami

Twenty-three strains of Staphylococcus aureus with borderline resistance to oxacillin were studied. These strains were not detected by the cefoxitin test, tests for penicillin-binding protein 2a (PBP2a), mecA, and mecA(LGA251) were negative, and the strains were genetically unrelated. To detect all strains resistant to oxacillin, laboratories should routinely test for both cefoxitin and oxacillin.

Journal: :Antimicrobial agents and chemotherapy 1990
K Ubukata R Nonoguchi M D Song M Matsuhashi M Konno

A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains hybridized to the mecA gene from Staphylococcus aureus; however, the chromosomal HindIII fragments containing the mecA genes were 3.4 kilobases in S. haemolyticus and 4.3 kilobases in S. simulans.

Journal: :Journal of clinical microbiology 2002
Flavia Huygens Graeme R Nimmo Jacqueline Schooneveldt Wendy J Munckhof Philip M Giffard

The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region.

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