Hydroxyapatite (HA) is the main inorganic component of bone and teeth having high bioactivity, biocompatibility osteointegration capability. Recently, wollastonite (WT) has been offered to reinforce HA further increase also mechanical strength. The focus this study was produce characterize HA/WT composite granules with spray drying. Commercial WT particles were introduced into lab made nanopart...

We used total internal reflection fluorescence microscopy to study quantitatively the motion and distribution of secretory granules near the plasma membrane (PM) of living bovine chromaffin cells. Within the approximately 300-nm region measurably illuminated by the evanescent field resulting from total internal reflection, granules are preferentially concentrated close to the PM. Granule motion...

Electron microscopic autoradiography with [3H]histidine, [3H]cystine, I13H]arginine, and [3H]proline was used to study protein synthesis in keratohyaline granules of newborn rats. All 3H-amino acids were incorporated into proteins in the granular cells, and the radioactive proteins appeared in the keratohyaline granules. However, the amount of radioactivity associated with the granules and the ...

Flaxseed (Linum usitatissimum L.) is the seed of a multipurpose plant pharmaceutical interest, as its mucilage can be used natural matrix to develop extended-release dosage forms and potentially replace synthetic polymers. In this study, 3² factorial design with two replicates central point was applied optimize development granules metformin HCl. The total fiber content well friability dissolut...

The neutrophil plays a central role in the innate host immune defense. Regulated exocytosis of its granules and release of antimicrobial and cytotoxic substances are key events to limit the spread of pathogens. However, the molecular mechanisms that control exocytosis of neutrophil granules are ill-defined. Recently, it was shown that Munc13-4 is essential for the priming of granules in several...

Chromogranin B (CgB, secretogranin I) is a secretory granule matrix protein expressed in a wide variety of endocrine cells and neurons. Here we generated transgenic mice expressing CgB under the control of the human cytomegalovirus promoter. Northern and immunoblot analyses, in situ hybridization and immunocytochemistry revealed that the exocrine pancreas was the tissue with the highest level o...

Isolated human skin mast cells (HSMC) were prepared and cultured overnight before functional and electron microscopic studies. Mast cell suspensions were examined after stimulation with anti-IgE to produce anaphylactic degranulation or examined in buffer-incubated controls. Histamine release was measured in replicate samples. Control, isolated HSMC studied by electron microscopy were well prese...

A partially purified secretory granule fraction, isolated from rat islets of Langerhans by differential centrifugation, was used for investigating the stability of the beta granules during incubation in various conditions . Effects of pH, temperature, and time were studied ; the granules possessed optimal stability at 4° and pH 6 .0, and could be solubilized at pH 4.0 or 8 .5, or in the presenc...