A polymerase chain reaction (PCR) method was developed that was capable of detecting a wide range of medically important fungi from clinical specimens. The primer pair was designed in conserved sequences of 18S-ribosomal RNA genes shared by most fungi. The lower limit of detection of this PCR technique was 1 pg of Candida albicans genomic DNA by ethidium bromide staining and 100 fg after Southe...

the objective of this study was to investigate fungal flora of the hair coat of domestic golden hamsterswith and without skin lesions. sixty hamsters were examined in the small animal teaching hospitalof the faculty of veterinary medicine, ferdowsi university of mashhad, and mashhad, iran. asteriletoothbrush was used for sample collection, from the entire body of the hamsters, all skin lesions ...

The lipophilic yeast Malassezia is an exacerbating factor in atopic dermatitis (AD) and colonizes the skin surface of patients with AD. With the goal of reducing the number of Malassezia cells, we investigated the antifungal activities of a therapeutic agent for AD, tacrolimus, and the azole agents itraconazole and ketoconazole against Malassezia species in vitro. We examined 125 strains of the...

BACKGROUND Malassezia species normally colonize the skin but they can change their saprophytic state and invade the stratum corneum as pathogens. AIMS To determine the prevalence of Malassezia species isolated from patients with pityriasis versicolor (PV) and to analyse their distribution according to the location of the lesion on the body. METHODS This study included 218 patients with PV a...

Penyakit infeksi jamur pada kulit kerap ditemukan daerah iklim tropis seperti di Indonesia, salah satunya adalah penyakit panu (Pityriasis versicolor) yang disebabkan oleh superfisialis Malassezia spp. Penularan terjadi apabila kontak dengan spora penyebabnya. Salah satu kelompok rentan terinfeksi ini yaitu pekerja bangunan. Meskipun tidak berbahaya tetapi keluhan rasa gatal waktu berkeringat d...

The genus Malassezia comprises commensal yeasts on human skin. These yeasts are involved in superficial infections but are also isolated in deeper infections, such as fungemia, particularly in certain at-risk patients, such as neonates or patients with parenteral nutrition catheters. Very little is known about Malassezia epidemiology and virulence. This is due mainly to the difficulty of distin...

the present study aimed at detection and species-level identification of the malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds a...